GOLDENSEAL(HYDRASTIS CANADENSIS)REMEDY FOR ORAL DISEASES

描述(申请人摘要)：补充和替代医学 (CAM)最近在美国公众中很受欢迎。研究 验证的CAM主要集中在系统性肺炎的治疗和预防上 对口腔疾病的关注较少，而对医疗疾病的关注较少。口头的 包括龋齿和牙周病在内的疾病是 失去工作和上学的天数。化学和机械手段已经被用来 控制牙菌斑细菌，龋病和牙周的病原体 疾病。然而，没有一种可用的药物是理想的，而且经常导致 不良影响。这证明了进一步寻找和开发替代方案的合理性 来自安全有效的天然来源的制剂。 北美植物加拿大水龙耳属植物，已知 在商业上被称为“黄金海豹”，几个世纪以来一直被用作防腐剂 治疗皮肤病，止泻，防腐，收敛， 止血剂和血管收缩剂。黄花海豹是主要的 年在保健食品店和药房出售的植物药物(“草药”) 美国的几种含黄花的口香糖和牙膏是 在市场上有售。尽管制造商已经提出了索赔 关于它对抗牙周病和预防龋齿的能力，没有科学的 有数据可以证实这些说法。建议的目标是 研究旨在评估黄花丹作为预防药物的潜力 以及治疗口腔疾病和维持口腔健康。这是假设的 对人类安全的抗菌化合物可以从H. 加拿大人。这些化合物可能有潜在的牙科用途。 预防/治疗药物，也可以作为先导化合物 随后设计和合成的新试剂甚至比 现有的那些。本研究的具体目的是：SA1：分离和 活性导向法鉴定加拿大毛细管藻中抗菌活性物质 分级和表征；SA2：测定其抗菌活性 抗龋齿和牙周病原体的纯化化合物；SA3：TO 研究从加拿大毛细管藻中分离纯化的抗菌化合物的混合物 协同抗菌活性；SA4：关联各种不同的生物活性 市面上可买到的含黄花丹的口腔卫生产品 在SA2中鉴定出的活性生物碱。 这项拟议的研究具有创新性，因为它代表了 一名口腔微生物学家和一名天然产品化学家将确保 黄花丹中新的或已知的活性化合物的快速发现 就药物的疗效提供科学的解释。它还将服务于 作为评估其口服现有草药的模型系统 与健康相关的索赔。本文对CAM的应用研究将有助于实现 更好的口腔健康和口腔疾病预防，是以下优先领域之一 美国公共卫生服务中心在《健康人2000》中明确了重点。

DESCRIPTION (APPLICANT'S ABSTRACT): Complementary and alternative medicine (CAM) has recently gained popularity with the American public. Research validating CAM has focused mainly on the treatment and prevention of systemic medical diseases while less attention has been paid to oral diseases. Oral diseases including dental caries and periodontal disease, are a major cause of loss of work and school days. Chemical and mechanical means have been used to control dental plaque bacteria, the etiologic agent of caries and periodontal disease. However, none of the available agents is ideal and frequently cause adverse effects. This justifies further search and development of alternative agents from natural sources that are safe and effective. The North American plant, Hydrastis canadensis L. (Ranunculaceae), known commercially as "Goldenseal," has been used for centuries as an antiseptic to treat skin disorders and as an antidiarrheal, antiseptic, astringent, hemostatic, and vasoconstrictor agent. Goldenseal is one of the major phytomedicines ("herbal remedies") sold in health food stores and pharmacies in the U.S. Several mouthrinses and toothpastes containing Goldenseal are available on the market. Although claims have been made by the manufactures regarding its ability to fight gum diseases and prevent caries, no scientific data is available to substantiate these claims. The goal of the proposed research is to evaluate the potential of Goldenseal as a remedy in prevention and treatment of oral diseases and to maintain oral health. It is hypothesized that antimicrobial compounds that are safe for humans can be identified from H. canadensis. These compounds may have potential as dental prophylactic/therapeutic agents and may also serve as lead compounds for the subsequent design and synthesis of new agents that are even more effective than the existing ones. The Specific Aims of this study are: SA1: To isolate and identify active antimicrobial compounds from H. canadensis by activity-guided fractionation and characterization; SA2: To determine antimicrobial activity of the purified compounds against cariogenic and periodontal pathogens; SA3: To investigate mixtures of purified antimicrobial compounds from H. canadensis for synergistic antimicrobial activities; SA4: To correlate bioactivity of various commercially available Goldenseal-containing oral hygiene products with levels of active alkaloids identified in SA2. The proposed research is innovative in that it represents collaboration between an oral microbiologist and a natural product chemist that will assure the speedy discovery of novel or known active compounds from Goldenseal and will provide scientific explanation as to the remedy's efficacy. It will also serve as a model system for the evaluation of existing herbal remedies for their oral health related claims. This application of CAM research will help to achieve better oral health and oral disease prevention, one of the to priority areas of focus specified b the U.S. Public Health Service in "Health People 2000."