HIGH RESOLUTION DATA ON DIFFERENT CDK2 CKS COMPLEXES

CKS细胞周期调节蛋白(Cks Hs1和Cks Hs2 in 人，分裂酵母中的Suc1和芽殖酵母中的CKS1)与 细胞周期蛋白依赖性激酶的催化亚单位(CDKs作为CDK2或CDK2) Cdc2)，对体内细胞周期进程是必不可少的。我们有 最近在两种不同的晶体中结晶了CDK2-Cks Hs1络合物 表格。这种复合体在较高分辨率下的结构至关重要。 以了解CKS的功能。因为CDK2与人CKS或 酵母同系物Suc1或CKS1，我们还计划将CDK2与一 酵母CKS蛋白的表达，以证明这些蛋白具有良好的特异性 蛋白质对CDKs的作用。

The CKS cell cycle regulatory proteins (CksHs1 and CksHs2 in human, suc1 in fission yeast and CKS1 in budding yeast) bind to the catalytic subunit of the cyclin-dependant kinases (Cdks as CDK2 or cdc2) and are essential for cell cycle progression in vivo. We have recently crystallized the CDK2-CksHs1 complex in two different crystal forms. A structure of this complex at higher resolution is critical to understand the Cks function. As CDK2 binds either human CKS or yeast homologs suc1 or CKS1, we also plan to crystallize CDK2 with one of the yeast CKS proteins to demonstrate the fine specificity of these proteins towards Cdks.