INTERLEUKIN 10 RECEPTOR COMPLEX

描述（改编自研究者摘要）：细胞因子 白细胞介素-10（IL-10）对免疫系统具有广泛的作用， 系统从抑制到刺激各种功能和细胞。 鉴定并克隆了一条结合IL-10的受体链（IL-10 R1）， 然而，这种单一受体链的存在不能解释 IL-10的所有作用。因此，我们着手确定第二个 链的IL-10受体复合物，并能够定义和 首先表征第二条链，命名为IL-10 R2。而 每条链单独不能赋予细胞对IL-10的完全应答性， 两个链的组合可以。随着识别的 第二链，IL-10 R2，一些关于功能的问题， 现在可以接近IL-10受体复合物。该提案旨在 回答关于IL-10 R2链在介导 IL-10在免疫系统和某些癌症中的作用整体 这一提议的基础假设是，两条受体链都是 IL-10的功能。结果将使我们能够理解 IL-10在免疫系统中的作用此外，由于爱泼斯坦- 巴尔病毒（EBV）产生称为vIL-10的IL-10的病毒同系物， 我们将检测IL-10和vIL-10之间的活性差异， 这将有助于深入了解vIL-10在EBV相关疾病中的作用， 恶性肿瘤。本研究的具体目的是：1）胡- IL-10功能中的IL-10 R2链; 2）确定IL-10如何抑制 其他细胞因子的功能; 3）IL-10 R2基因的调节; 4） 确定IL-10和IL-10受体链的mRNA的调节 （IL-10 R1和IL-10 R2）; 5）定义细胞免疫功能的差异， 和病毒IL-10。我们的研究结果应该确立第二个 链在IL-10受体中的功能，增强我们对IL-10如何 功能，并提供深入了解EBV蛋白vIL-10的作用 EB病毒诱发的癌症。此外，由于IL-10是一种重要的 免疫抑制细胞因子在免疫功能中，例如在 T细胞亚群，所获得的知识应该有助于我们了解 IL-10介导免疫抑制的机制。

DESCRIPTION (Adapted from Investigator's Abstract): The cytokine interleukin-10 (IL-10) exhibits a wide spectrum of effects on the immune system from inhibition to stimulation of various functions and cells. One receptor chain that binds IL-10 was identified and cloned (IL-10R1), however, the existence of this single receptor chain could not explain all the effects of IL-10. Accordingly, we set out to identify the second chain of the IL-10 receptor complex and were able to define and initially characterize the second chain, designated IL-10R2. Whereas each chain alone cannot impart full responsiveness of cells to IL-10, the combination of both chains can. With the identification of the second chain, IL-10R2, a number of questions regarding the functional IL-10 receptor complex can now be approached. This proposal is designed to answer questions about the role of the IL-10R2 chain in mediating IL-10 effects in the immune system and in some cancers. The overall hypothesis underlying this proposal is that both receptor chains are required for function of IL-10. The results will enable us to understand how IL-10 functions in the immune system. In addition, since Epstein- Barr virus (EBV) produces a viral homolog of IL-10 designated vIL-10, we will examine the differences in activity between IL-10 and vIL-10 that should provide insight into the role of vIL-10 in EBV-related malignancies. Specific aims of this study are: 1) the role of the Hu- IL-10R2 chain in IL-10 function; 2) determine how IL-10 inhibits functions of other cytokines; 3) regulation of the IL-10R2 gene; 4) determine regulation of the mRNAs of IL-10 and the IL-10 receptor chains (IL-10R1 and IL-10R2); 5) define the differences in function of cellular and viral IL-10. Our results should establish the role of the second chain in IL-10 receptor function, enhance our understanding of how IL-10 functions, and provide insight into the role of the EBV protein vIL-10 in EBV-induced cancers. Furthermore, because IL-10 is an important immunosuppressive cytokine in immune function such as in development of T-cell subsets, the knowledge gained should help us understand the mechanisms by which IL-10 mediates immunosuppression.