MATRIX AND HIV1 GAG TARGETING

描述：(改编自《调查者摘要》)此应用程序 提出研究GAG如何到达血浆这一重要问题 薄膜。P.I.S实验室的初步研究表明，随着 肉豆蔻化信号MA的例外对于 高效的病毒颗粒生产和MA核心亚区主要 控制GAG膜结合的特异性而不是效率。 初步数据还表明，缺乏高达90%MA的突变体可以 如果Env掺入的缺陷是 对，是这样。这项研究的目标是(目标1)确定 对GAG膜靶向的动力学和特异性；对 HIV病毒粒子膜不同的脂类成分；与 带着细胞骨架的马。在AIM 2中，肉豆蔻酸化和MA的作用 N-末端肉豆蔻醇的膜插入调控中的磷酸化 MA组(万花柱转换假说)将使用以下方法进行检验 复制能力强的MA删除的病毒和其他突变体作为工具。

DESCRIPTION: (Adapted From Investigator's Abstract) This application proposes to study the important question of how Gag reaches the plasma membrane. Preliminary studies in the P.I.'s laboratory suggest that, with the exception of the myristylation signal, MA, is entirely dispensable for efficient viral particle production and that the MA core subdomain primarily governs the specificity rather than the efficiency of Gag membrane binding. Preliminary data also indicate that mutants which lack up to 90% of MA can replicate efficiently in certain cells if a defect in Env incorporation is correct. The goals of the study are (AIM 1) to determine the contribution of MA: to the kinetics and specificity of Gag membrane targeting; to the distinct lipid composition of the HIV virion membrane; and to association of MA with the cytoskeleton. In AIM 2, the role of myristylation and MA phosphorylation in regulating membrane insertion of the N-terminal myristyl group of MA (the myristyl switch hypothesis) will be examined using replication-competent MA-deleted viruses and other mutants as tools.