DEREGULATION OF CELL GROWTH BY K1 GENE OF KAPOSIS SARCOMA ASSOCIATED HERPESVIRUS

在一个与编码塞米里鱼的基因相同的位置上 松鼠猴疱疹病毒（HVS）转化蛋白（STP） 肉瘤相关疱疹病毒（KSHV）包含一个独特的开放阅读 虽然KSHV和HVS是K1的相关成员， γ疱疹病毒的杆状病毒亚组，K1和STP不表现出 氨基酸序列或结构组织的相似性， STP是需要的致癌潜力的HVS，我们调查 K1表达的功能后果K1基因的表达 在啮齿类成纤维细胞中产生的形态学变化和病灶形成 一种重组疱疹病毒，其中， 将HVS的STP癌基因替换为K1， 淋巴细胞以IL-2非依赖性生长和诱导淋巴瘤常见 这些结果证明了绒猴的转化潜力。 KSHV的K1基因出版物Lee，H，Veazey，R，威廉姆斯，K，Li，M， Guo，J，Neipel，F，Fleckenstein，B，Lackner，A，Desrosiers，RC，和 Jung，JU卡波西氏病K1基因对细胞生长的失调 肉瘤相关疱疹病毒自然医学1998; 4：435 - 440

At a position equivalent to the gene encoding the saimiri transforming protein (STP) of herpesvirus saimiri (HVS), Kaposi's sarcoma-associated herpesvirus (KSHV) contains a distinct open reading frame called K1 Although KSHVand HVS are related members of the rhadinovirus subgroup of gamma herpesviruses, K1 and STP exhibit no similarity in amino acid sequence or in structural organization Since STP is required for the oncogenic potential of HVS, we investigated the functional consequence of K1 expression Expression of the K1 gene in rodent fibroblasts produced morphologic changes and focus formation indicative of transformation A recombinant herpesvirus in which the STP oncogene of HVS was replaced with K1, immortalized primary T lymphocytes to IL-2 independent growth and induced lymphoma in common marmosets These results demonstrate the transforming potential of the K1 gene of KSHV PUBLICATIONS Lee, H, Veazey, R, Williams, K, Li, M, Guo, J, Neipel, F, Fleckenstein, B, Lackner, A, Desrosiers, RC, and Jung, JU Deregulation of cell growth by the K1 gene of Kaposi's sarcoma-associated herpesvirus Nature Med 1998; 4:435-440