STRUCTURAL STUDIES OF CYTOCHROME P450

细胞色素P450是一种参与解毒的酶， 过程 细菌酶已被纯化至同质， 在SDS-PAA凝胶上以100 kDa蛋白运行。 布莱克博士和同事们 已经能够获得小的三维晶体， 对于X射线晶体学分析来说不够大。 超离心实验表明了一个不寻常的轴比 这个分子的比例是7：1 以方便任何结构工程，并 为了验证轴比，Black博士访问了我们的实验室， 蛋白质冷冻电镜。 初步图像显示 支撑细长形状的模型。 另外这款 已知水溶性蛋白质与脂质相互作用，可能是一种 外周膜蛋白 因此，已经开始进行实验 使细胞色素P450在脂质单层上结晶。

Cytochrome P450 is an enzyme involved in the detoxification process. The bacterial enzyme has been purified to homogeneity and runs as a 100 kDa protein on SDS-PAA gels. Dr. Black and co-workers have been able to obtain small three-dimensional crystals which are not sufficiently large for x-ray crystallographic analysis. Ultracentrifugation experiments have indicated an unusual axial ratio of 7:1 for this molecule. To facilitate any structural work and to verify the axial ratio, Dr. Black visited our lab to investigate the protein by electron cryo-microscopy. Preliminary images seem to support the model of an elongated shape. In addition, this water-soluble protein is known to interact with lipids, perhaps as an peripheral membrane protein. Experiments have therefore been started to crystallize the cytochrome P450 on lipid monolayers.