Microstructural Dynamics of Reticulocyte Membranes

在红细胞生成结束时，红细胞排出它们的核以成为 网织红细胞。在这个过程中，质膜组分的分配 在突出的核和年轻的网织红细胞之间是由 膜和骨架网络的分子组织。中美之间的差异 网织红细胞和红细胞的形态和力学性质表明 网织红细胞成熟过程中膜结构的变化。是这样的 组织变化也可能导致粘连功能的丧失 红细胞中的Lu、Lw和CD47等分子。我们对此知之甚少 幼龄网织红细胞膜和骨骼的分子组织 网络。该提案旨在应用各种技术，包括 蛋白质电泳、蛋白质印迹分析。免疫荧光显微镜， 细胞培养和单细胞微吸管操作，以研究 膜和骨骼分子的分子组织和动力学 幼龄网织红细胞及其特异性配基对细胞膜的影响 骨架网络的组织和力学特性。

At the conclusion of erythropoiesis, erythroblasts expel their nuclei to become reticulocytes. In this process, partitioning of plasma membrane components between the extruded nucleus and young reticulocyte is governed by the molecular organization of the membrane and skeletal network. The differences in morphology and mechanical properties of reticulocytes and erythrocytes suggest alterations of membrane organization during reticulocyte maturation. Such organizational changes may also cause the loss of function of adhesion molecules, such as Lu, LW and CD47, in erythrocytes. Little is known regarding the molecular organization of the young reticulocyte membrane and skeletal network. This proposal is designed to apply various techniques, including protein electrophoresis, Western blot analysis. immunotluorescence microscopy, cell culture, and single cell micropipette manipulation, to investigate the molecular organization and dynamics of membrane and skeletal molecules in the young reticulocyte and the effect of specific liganding on membrane organization and mechanical properties of the skeletal network.