Effects of oxidative stress on glial cell membranes

氧化应激对神经胶质细胞膜的影响

• 批准号: 7337080
• 负责人: JAMES C LEE
• 金额: $ 16.09万
• 依托单位: UNIVERSITY OF MISSOURI-COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7337080
• 关键词: Alzheimer' s Disease; Amyloid; Antibodies; Astrocytes; Authorization documentation; Binding; Biochemical; CD36 gene; CD47 gene; Cell Line; Cell membrane; Cell physiology; Cells; Cytoskeleton; Cytosolic Phospholipase A2; Disclosure; Elements; Environment; Face; Feedback; Gel; Human Resources; Image; In Situ; Inflammation; Inflammatory Response; Instruction; Last Name; Lead; Limes; Lipid Peroxidation; Localized; MAP Kinase Gene; MAPK14 gene; Maps; Measurement; Membrane; Microglia; Microscopy; Missouri; NADPH Oxidase; Names; Neuroglia; Numbers; Oxidative Stress; Pathogenesis; Pathway interactions; Phase; Principal Investigator; Printing; Property; Proteins; Reactive Oxygen Species; Registries; Reporting; Research; Research Personnel; Research Project Grants; Role; SR-A proteins; Signal Transduction; Techniques; Testing; Universities; fluorescence imaging; human embryonic stem cell; laurdan; novel; oxidation; peptide A; programs; receptor

The objective of this proposal is to investigate the roles of membrane factors in amyloid-p peptide (A(3)-induced oxidative stress and inflammatory responses in glial cells. Here membrane factors include local membrane phase properties and cytoskeletal linkage of membrane receptors. Ap-induced oxidative stress and inflammation are implicated in Alzheimer's disease (AD). In fact, Ap has been found to induce oxidative stress through activation of NADPH oxidase. Excess reactive oxygen species (ROS) in cells, in turn, cause oxidative damage including lipid peroxidation, oxidation of RNA, DMA, and proteins, which subsequently perturb normal cellular processes, including intracellular signaling and cytoskeleton organization. In this regard, we have previously reported that oxidative stress causes astrocyte membrane to become more gel- like through activations of p38 MARK and of cytosolic phospholipase A2 (cPLA2). Our preliminary results also show Ap42 oligomers induce activation of cPLA2. Since it has been reported that the efficiency of NADPH oxidase activation is dependent of its local membrane environment and our preliminary studies show that the membrane subunit of NADPH oxidase, gp91ph0*, is predominately localized at the high GP domains (i.e. more gel-like membranes) in astrocytes, these findings lead us to hypothesize that Af!*? induces cPLA? activation through activations of NADPH oxidase and MAPK pathways to cause glial membranes to become more gel-like, which, in turn, becomes a positive feedback to further amplify the activation of NADPH oxidase to produce ROS. Other membrane factors, such as cytoskeletal linkages of membrane receptors, can also be a fundamental element governing cell functions. It has been reported that Ap42 binds to membrane receptors, CD36, ctePi, CD47 and scavenger receptor class A, resulting in inflammatory responses in microglial cells, which can be suppressed by blocking the binding of Ap42 to one of these receptors using their antibodies. These findings lead us to hypothesize that cooperativitv between these membrane receptors is required for AB-induced inflammatory responses in microglial cells and this coooerativitv is established through the cvtoskeletal linkages of these membrane receptors. Since oxidative stress and inflammation are implicated in AD, our study on how membrane factors involved in the mechanisms of Ap-induced oxidative stress and inflammatory responses in glial cells will prove critical to deepen our understandings in the pathogenesis of AD. Novel biophysical techniques including fluorescence imaged deformation (FIMD) and fluorescent microscopy of LAURDAN, and various biochemical techniques will be applied to accomplish this proposed project.

本研究的目的是探讨膜因子在A(3)诱导的神经胶质细胞氧化应激和炎症反应中的作用。在这里，膜因素包括局部 膜受体的膜相特性和细胞骨架连接。AP诱导的氧化应激和炎症与阿尔茨海默病(AD)有关。事实上，AP已被发现能诱导氧化 通过激活NADPH氧化酶应激。细胞中过量的活性氧物种(ROS)反过来会导致氧化损伤，包括脂质过氧化、RNA、DMA和蛋白质的氧化，从而导致 扰乱正常的细胞过程，包括细胞内信号和细胞骨架组织。在这方面，我们之前已经报道过，氧化应激导致星形胶质细胞膜变得更凝胶- 如通过激活p38Mark和胞浆磷脂酶A2(CPLA2)。我们的初步结果还表明，Ap42寡聚体可以诱导cPLA2的激活。因为有报道称， NADPH氧化酶的活性依赖于其局部的膜环境，我们的初步研究表明，NADPH氧化酶的膜亚单位gp91ph0*主要定位于高GP 结构域(即更多的凝胶样膜)，这些发现引导我们假设Af！*？导致中国人民解放军？通过激活NADPH氧化酶和MAPK通路，使胶质细胞膜变得更像凝胶一样，这反过来成为一个正反馈，进一步放大NADPH氧化酶的激活，产生ROS。其他膜因子，如膜受体的细胞骨架连接，也可以是 控制细胞功能的基本要素。据报道，Ap42与膜受体CD36、ctePI、CD47和清道夫受体A类结合，在小胶质细胞中引起炎症反应。 它可以通过使用这些受体的抗体阻断Ap42与其中一个受体的结合而被抑制。这些发现使我们假设，这些膜受体之间需要协同作用 AB诱导小胶质细胞的炎症反应，这种反应是通过这些膜受体的细胞骨架联系建立的。由于AD与氧化应激和炎症反应密切相关，因此研究膜因子在AP诱导的神经胶质细胞氧化应激和炎症反应中的作用机制，对于加深对AD发病机制的认识具有重要意义。新的生物物理技术，包括Laurdan的荧光成像变形(FIMD)和荧光显微镜，以及各种生化技术将被应用来完成这一拟议的项目。

项目成果

Nanoparticle-emitted light attenuates amyloid-β-induced superoxide and inflammation in astrocytes.

• DOI: 10.1016/j.nano.2013.10.007
• 发表时间: 2014-01
• 期刊: NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
• 影响因子: 5.4
• 作者: Bungart, Brittani L.;Dong, Li;Sobek, Daniel;Sun, Grace Y.;Yao, Gang;Lee, James C-M.
• 通讯作者: Lee, James C-M.