Biogratings for Combinatorial Library Characterization

描述(由申请人提供)：如果具有巨大多样性的文库能够在初步的几轮筛选中被表征为生物活性，那么高通量筛选的效率可以被提高。目前还没有能够表征组合文库生物活性的仪器，为了快速表征组合文库的生物活性，智能光学系统(LOS)提出了一种基于无标记检测原理的新型、廉价的光学仪器。建议的仪器将具有与表面等离子体共振相当的灵敏度，并将实时测量结合事件。该仪器将能够区分高亲和力的特异性结合和低亲和力的非特异性结合。该装置由一个激光光源、一个生物芯片、一个流动池、一对探测器和一台分析探测器输出的计算机组成。在第一阶段，LOS将通过稳定的蛋白质-蛋白质相互作用监测、瞬时酶-底物反应和小分子蛋白质相互作用来测试该仪器的性能，从而证明将该仪器用作表征组合文库生物活性的工具的可行性。

DESCRIPTION (provided by applicant): The efficiency of high throughput screening can be improved if libraries with huge diversity could be characterized for bioactivity in preliminary rounds of screening. There are currently no instruments that can characterize the bioactivities of combinatorial libraries.Intelligent Optical Systems (lOS) proposes to develop a new, inexpensive, optical instrument, based on a label less detection principle, for the rapid characterization of the bioactivities of combinatorial libraries. The proposed instrument will have sensitivities comparable to surface plasmon resonance and will measure binding events in real time. The instrument will be able to distinguish high affinity specific interactions from low affinity nonspecific binding. The proposed device consists of a laser light source, a biochip, a flow cell, a pair of detectors, and a computer for analyzing the detector's output. In Phase I, lOS will demonstrate the feasibility of using this instrument as a tool for characterizing the bioactivity of combinatorial libraries by testing the performance of the instrument through stable protein-protein interaction monitoring, transient enzyme-substrate reactions, and small molecule protein interaction.