FISH ASSAY FOR THE DETECTION OF T (14;18) (Q32;21)

描述（申请人摘要）：大约60%的B细胞 淋巴瘤的特征在于存在t（14;18）（q32;q21） 染色体易位诊断和治疗后随访目前 通过核型分析、Southern印迹或PCR进行，这些方法存在以下问题 特异性和灵敏度。荧光原位杂交 解决了这些局限性;然而，没有临床测试的FISH探针， 可用于检测t（14; 18）易位。第一阶段研究已完成 用于t（14; 18）易位间期FISH分析的新探针。使用 正常和肿瘤样品，高灵敏度和特异性的探针 观察了使用来自多种组织类型的肿瘤标本，II期 研究将验证探针用于诊断的用途。第二阶段研究将 并验证其用于监测微小残留病。在第二阶段 研究，探针也将优化，以减少背景杂交 信号了 拟议商业应用：不可用

DESCRIPTION (applicant's abstract): Approximately 60 percent of B-cell lymphomas are characterized by the presence of the t(14;18) (q32;q21) chromosomal translocation. Diagnosis and post-treatment follow-up are currently performed by karyotype, Southern blotting, or PCR, which have problems with specificity and sensitivity. Fluorescence in situ hybridization (FISH) addresses these limitations; however, no clinically tested FISH probes are available for detecting the t(14; 18) translocation. Phase I research developed novel probes for interphase FISH analysis of the t(14; 18) translocation. Using both normal and tumor samples, high sensitivity and specificity of the probes were observed. Using tumor specimens from multiple tissue types, Phase II research will validate use of the probes for diagnosis. Phase II research will also validate their use for monitoring minimal residual disease. In Phase II research, the probes will also be optimized to reduce background hybridization signal. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE