Functional Imaging of the Vascular Bed

描述（由申请人提供）：我们小组最近推出了实时3d成像技术，能够在不需要造影剂的情况下研究血管床的功能特性。这种技术被称为动态近红外光学断层扫描（DYNOT），采用无害的近红外光源，便携，适合长时间监测，适用于各种临床环境（包括床边和术中）。在这项研究中，我们计划研究这项技术在早期检测糖尿病易感性个体、非胰岛素依赖型糖尿病（NIDDM）患者和胰岛素依赖型糖尿病（IDDM）患者和晚期微血管病变明显症状的肢体微血管病变和神经病变中的应用。计划中的研究旨在验证这样一种假设，即通过分析从受试者前臂和小腿收集的时间序列成像数据确定，微血管的基线时频、自动调节和血管反应性反应的可测量差异与疾病的严重程度相关。成像研究将包括进行双波长（760和830纳米）层析成像测量，每次图像帧率为3 Hz，最长20分钟。这一假设的验证提供了表征糖尿病对外周效应机制的早期影响的潜力，从而为改进无创监测和治疗方案的发展奠定了基础。

DESCRIPTION (provided by applicant): Our group has recently introduced working real-time 3D-imaging technology capable of investigating the functional properties of the vascular bed without the need of contrast agents. This technology, referred to as Dynamic Near-infrared Optical Tomography (DYNOT), employs harmless near infrared light sources, is portable, suitable for prolonged monitoring, and is adaptable to a wide variety of clinical environments (including bedside and intraoperative). In this study, we plan to investigate the utility of this technology to detect, early-on, microangiopathy and neuropathy in the limbs of individuals genetically predisposed to contract diabetes, those with non-insulin-dependent diabetes (NIDDM) and those with insulin-dependent diabetes (IDDM) and overt symptoms of advanced microangiopathy. Planned studies are designed to test the hypothesis that measurable differences in the baseline time-frequency, auto regulatory and vasoreactivity responses of the microvasculature will correlate with the severity of disease as determined from analysis of time-series imaging data collected from the forearms and calfs of subjects. Imaging studies will involve performing dual wavelength (760 and 830 nm) tomographic measures at an image-framing rate of 3 Hz for up to 20 minutes per session. Validation of this hypothesis offers the potential to characterize the earliest effects of diabetes on the peripheral effector mechanism, thereby setting the stage for development of improved noninvasive monitoring and treatment protocols.