INFORMATICS CENTER FOR MOUSE NEUROGENETICS

说明(申请人摘要)：目的 神经信息学项目是开发和利用一套图像数据库， 电动互联网显微镜和研究遗传基础的软件 小鼠中枢神经系统的结构变异。研究资源是开放的 通过集成的Web界面访问&lt；nervenet.org&>社区。的关注点 该项目旨在为地图绘制提供一个协作研究环境 数量性状座位(QTL)。这些基因是导致 小鼠和人类中枢神经系统结构的异常差异。QTL分析是 一个新兴的领域，研究由许多人调节的复杂的生物特征 基因。我们将开发四项重要的新资源和技术：(1) 小鼠脑库(MBL)由一个庞大的、组织良好的脑库组成 适合进行形态测量研究的切片。数以千计的图像可以 以每像素5微米的分辨率快速搜索、排序和下载 使用直观而强大的Web界面。(2)互联网显微镜 系统(IScope)捕获并显示极其详细的电影-z轴图像 STACKS-适合对MBL中的所有大脑进行复杂的体视学研究。 IScope包括24小时通过网络控制的机器人幻灯片操控器 一周7天每天工作(3)神经制图员项目将开发一套 软件工具和数百个神经解剖结构的3D模型， 将使研究人员能够重建和数字解剖；材料在 MBL.(4)神经遗传学工具箱(NTB)包括一套基因图谱 使神经科学家能够快速识别和评估QTL的程序 负责CNS体系结构的惊人变化。NTB将会 包括来自异常大高级杂交的基因类型，该杂交设计用于定位 具有足够精确度的基因座，使候选基因的克隆成为可能 QTL。实现这四个项目的目标将催生 对成年哺乳动物神经系统的结构分析，并将导致 大量关于发育、衰老和 人脑的病理学。

DESCRIPTION (Applicant's Abstract): The purpose of this Neuroinformatics project is to develop and exploit a suite of image databases, motorized Internet microscopes, and software to study the genetic basis of structural variation of the mouse CNS. Resources are open to the research community through an integrated web interface at <nervenet.org>. The focus of this project is to provide a collaborative research environment for mapping quantitative trait loci (QTLs). These genes are responsible for the extraordinary variation in CNS structure among mice and humans. QTL analysis is a burgeoning field that tackles complex biological traits modulated by many genes. We will develop four significant new resources and technologies: (1) The Mouse Brain Library (MBL) consists of a huge, well-organized library of brain sections suitable for morphometric investigation. Thousands of images can be rapidly searched, sorted, and downloaded at a resolution of 5 microns per pixel using an intuitive and powerful web interface. (2) The Internet Microscope System (iScope) captures and displays extremely detailed movies -z-axis image stacks-suitable for sophisticated stereological study of all brains in the MBL. The iScope includes robotic slide handlers controlled over the web 24 hours a day, 7 days a week (3) The NeuroCartographer Project will develop a suite of software tools and 3D models of hundreds of neuroanatomical structures that will enable researchers to reconstruct and digitally dissect; material in the MBL. (4) The Neurogenetics Tool Box (NTB) comprises a set of gene mapping programs that will enable neuroscientists to rapidly identify and evaluate QTLs responsible for the astonishing variation in CNS architecture. The NTB will include genotypes from an unusually large advanced intercross designed to map loci with sufficient precision to enable a candidate gene approach to cloning QTLs. Achieving the aims of these four projects will catalyze a new era in the structural analysis of the adult mammalian nervous system and will lead to a large number of novel lines of research on the development, aging, and pathology of the human brain.