MOLECULAR BASIS OF MALE INFERTILITY

本申请建议在贝勒医学院设立一个研究男性不育的项目，重点是调查精子产生异常的原因，并应用辅助生殖技术来克服这些缺陷。该计划项目将有一个核心行政单位作为其核心，该单位将协调基础和临床科学家的日常活动。该计划项目的重点将放在男性不育的分子生物学领域，特别强调定义正常精子发生所需的基因。辅助生殖技术已经被用来克服许多男性因素缺陷。人们认为，如果精子能受精，那么它就是“健康的”。我们没有这种看法的科学依据，而且这些后代中的许多人可能携带着基因缺陷。这项建议的目的是：1.建立一个男性不育研究项目，为来自泌尿外科、妇产科、细胞生物学和医学遗传学的基础和临床科学家之间建立密切的工作关系。将这些小组聚集在一个项目中，将比这些科学家单独工作时更有效地关注男性不育问题。2.建立一个分析DNA和进行原位杂交的设施是本计划项目的一个重要和关键的组成部分。3.将男性不育症的临床特点与MO水平的新发现联系起来，以更好地了解疾病的过程，从而开发更有效的治疗和诊断方法。这个多学科的科学家小组共同工作的专业知识有望对男性不育的基本病理生理学产生新的理解。

This application proposes the establishment of a Program Project at Baylor College of Medicine to study male infertility with a focus on investigation the etiology of abnormal sperm production and the application of assisted reproductive techniques to overcome these defects. The Program Project will have as its nucleus a core administrative unit that will coordinate the activities of the basic and clinical scientists on a day to day basis. The focus of this Program Project will be in the area of the molecular biology of male infertility with specific emphasis on defining the genes required for normal spermatogenesis. Assisted reproductive techniques have been used to overcome many male factor defects. It is assumed that if a sperm can fertilize, than it is "healthy". We have no scientific basis for this belief and many of these offspring could be carrying genetic defects. The aims for the proposal are: 1. To establish a program project for the study of male infertility creating a close working relationship between basic and clinical scientists from the Departments of Urology, Obstetrics and Gynecology., Cell Biology, and Medical Genetics. Bring these groups together in one project will create a more effective focus on male infertility than would occur with these scientists working individually. 2. To Create a facility to analyze DNA and perform in situ hybridization represents a significant a significant and critical component of this Program Project. 3. To correlate clinical features of male infertility with new discoveries at the mo level, to better understand the diseases processes and thereby to develop ad more effective treatment and diagnostic modalities. The expertise of this multi-disciplinary group of scientists working together is expected to yield new understanding of the basic pathophysiology of male infertility.