HERMES AND MARINER TRANSFORMATION OF AEDES AEGYPTI

病媒传播的疾病继续导致大量死亡， 世界各地的人的发病率。 而疟疾和黄色 发烧是欠发达国家的主要疾病，登革热的威胁 发烧是在美国的边界。控制传输 这些疾病可以通过控制其昆虫媒介来实现。 蚊子转基因技术的发展，使转基因蚊子的生产成为可能。 通过稳定插入而发生改变的蚊子 外源基因我们的长期目标是生产转基因蚊子 它们能抵抗病原体的感染， 用于研究病原体-载体相互作用以及释放生物体 用于寻求遗传控制寄生虫传播的项目， 和病毒性疾病。 此外，transmittance还可以 研究生物化学和分子生物学过程中涉及的基本 mosuito生理学方面如血液喂养和消化， 卵子发生和寻找宿主。 基于转座子的基因分析 蚊子有可能识别出许多关键基因， 表达对于载体能力和载体能力是重要的。 最近，我们已经证明，twso第二类转座因子，爱马仕， 和水手，介导外源DNA插入染色体 黄热病蚊子。埃及伊蚊（Jasinskiene等，一九九八年; 科茨等人，1998年）。 这些转换的进一步改进 需要系统来促进它们在生产病原体中的使用， 抗药性蚊子和其他菌株的基本生物 为达到这些目的，我们提出以下具体目标：1） 开发常规分离DNA片段的方法， 来自Hermes转化的蚊子品系的转座子-染色体连接; 2)制定爱马仕重新动员的程序和应变措施， Mariner染色体插入; 3）产生转基因菌株 利用cre-loxp和FLP-FRT位点特异性重组的蚊子 用于比较启动子分析。 成功完成这些 具体目标将导致强大和广泛适用的系统，或 蚊子转基因

Vector-borne diseases continue to cause significant mortality and morbidity in people throughout the world. While malaria and yellow fever are scourges of lesser-developed countries, the threat of Dengue fever is at the borders of the United States. Control of transmission of these diseases can be achieved by controlling their insect vectors. The development of mosquito transgenesis has made possible the producion of mosquitoes that have been altered by the stable insertion of exogenous genes. Our long-term goal is to produce transgenic mosquitoes that are resistant to infection by pathogens and use these as systems for studying pathogen-vector interactions as well as release organisms for programs seeking genetic control over the transmission of parasitic and viral diseases. Furthermoe, transormation makes it possible to study biochemical and molecular biological processes involved in basic aspects of mosuito physiology such as blood feeding and digestion, oogenesis and host seeking. Transposon-based genetic analysis of mosquitoes has the potential to identify many crucial genes whose expression is important for vector competence and vectorial capacity. Recently, we have shown that twso Class II transposable elements, Hermes and mariner, mediate the insertion of exogenous DNA into the chromosomes of the yellow fever mosquito. Aedes egypti (Jasinskiene et al., 1998; Coates et al., 1998). Further refinements of these transformation systems are reuqired to facilitate their use in producing pathogen- resistant mosquitoes and other strains for basic biological study.Towards these ends we propose the following specific aims: 1) develop methods for routine isolation of DNA fragments consisting of transposon-chromosome junctions from Hermes-transformed mosquito lines; 2) develop procedures and strains for remobilization of Hermes and Mariner chromosomal insertions; 3) produce transgenic strains of mosquitoes that exploit cre-loxp and FLP-FRT site-specific recombination for comparative promoter analysis. Successful accomplishment of these Specific Aim will result in robust and widely-applicable systems or mosquito transgenesis.