Cdna Microarrays In Gene Expression Of Uveitis Patients

cDNA微阵列已被应用于各种各样的研究，从调查某种类型的细胞的信号转导途径，以分析复杂的临床疾病的基础上差异基因表达模式。利用cDNA微阵列的研究为研究者提供了重要的新观察。为了深入了解葡萄膜炎（一种自身免疫性疾病）的分子机制，这种方法目前正在应用于观察小鼠实验性自身免疫性葡萄膜炎。我们建议使用相同的技术评价循环淋巴细胞和眼内细胞（如可用）。已经开发了具有12，000个基因的特异性免疫阵列以及其他几种独特的阵列膜，使我们能够使用标准化技术来分析患有明确的眼内炎性疾病的患者的基因表达模式。在人类中使用这些阵列将为研究人员提供基于差异基因表达模式来分析复杂疾病的机会。高选择性免疫阵列以及其他独特的阵列为我们提供了同时检查5000到12，000个基因的选择。我们希望通过cDNA微阵列分析，确定独特的基因表达谱，以及疾病相关基因的患者在确定的临床阶段的眼部炎症性疾病，从而提供进一步的见解，了解病理机制和潜在的治疗目标？本研究刚刚开始，没有可报告的数据。

cDNA microarrays have been applied to a wide variety of studies from investigating signal transduction pathways of a certain type of cells to profiling complex clinical disorders based on differential gene expression pattern. Studies using cDNA microarray have provided investigators with important new observation. To gain insight to molecular mechanisms of uveitis, an autoimmune disease, this approach is presently being applied to look at experimental autoimmune uveitis in mice. We propose evaluating circulating lymphocytes and intraocular cells(when available) using the same technique. A specific immune array with 12,000 genes , as well as several other unique array membranes have been developed permitting us to use a standardized technique to analyze gene expression pattern in patients with defined intra-ocular inflammatory diseases. The use of these arrays in humans will provides the investigator the chance to profile complex diseases based on differential gene expression patterns. The highly selective immune array as well as other unique arrays, provides us options to examine 5000 to 12,000 genes simultaneously. We wish to identify unique gene expression profiles as well as disease relevant genes for patients with ocular inflammatory disease at defined clinical stages by cDNA microarray analysis, therefore provide further insight to understanding pathological mechanisms and potential targets for treatment? This study is just beginning and no reportable data is available.