Engineering long-acting oral enzymes for animal nutrition through site-specific anchorage

磷是动物生长的关键矿物质营养物质。植酸是植物性饲料中磷的主要储存形式。然而，植酸中的磷对非反胃动物如家禽和猪是不可用的。解决动物饲料中有效磷含量低的方法之一是在饲料中添加植酸酶。植酸酶是一种从植酸中释放磷酸盐的酶。这个项目的目的是开发一种将植酸酶固定在肌胃中的方法，肌腱是植酸酶在胃肠道中的主要作用部位。我们假设，改善植酸酶在肌胃中的保留将提高酶的疗效。该项目的主要工作将是利用一个适应胃部环境的噬菌体展示平台，选择一种针对肌胃的结合蛋白。然后，选定的结合蛋白可以在基因上与植酸酶融合，并评估对酶效力的影响。目的是开发的平台可能会促进未来在胃肠道的靶向应用。伊布，西南部

Phosphorus is a key mineral nutrient for animal growth. Phytic acid is the main storage form of phosphorus in plant-based feeds. Phosphorus in phytic acid, however, is not available to non-ruminant animals such as poultry and swine. One solution to the problem of low available phosphorus in animal feed is supplementing feed with phytase. Phytase is an enzyme that releases phosphate from phytic acid. The aim of this project is to develop a means of anchoring phytase in the gizzard, its main site of action in the gastrointestinal tract. We hypothesize that improved phytase retention in the gizzard will improve the enzyme's efficacy. The main work of this project will be to select a binding protein specific to the gizzard using a phage display platform adapted to the gastric environment. The selected binding protein can then be genetically fused to phytase and the impact on the enzyme's efficacy assessed. It is intended that the platform developed may facilitate future targeting applications in the gastrointestinal tract. IBB, ENWW