Effect of amiloride and gurmarin on taste receptor cells

描述(由申请人提供)：建议的研究旨在 研究特定味觉输入到神经系统的排列 在感受器阵列中。眼前的目标是理解 阿米洛利和古尔马林敏感信号转导机制的分布 味觉感受器细胞(TrCs)位于菌状和腭部上皮中。 为了实现这些目标，选择了一种实验范式， 允许从基底膜记录跨膜电流 在味觉刺激后的原位个体TRC的数量 粘膜表面。因此，这一程序允许一个人化学刺激TRC 以一种代表这些细胞如何自然相互作用的方式 使用味觉刺激，这为测量调谐提供了一种公正的方法 储税券简介。此外，该制剂非常适合于测定 阿米洛利和古尔马林对大鼠味觉敏感性的影响 个别储税券。再加上已知的关于这些影响的信息 关于更高阶神经元元件的物质，从建议的 实验可能从逻辑上揭示特定突触联系的存在 在特定类型的trc和表达共同 在特定的味觉输入阻断后，敏感度发生变化。

DESCRIPTION (provided by applicant): The proposed research is designed to investigate the arrangement of specific gustatory inputs to the nervous system within the receptor array. The immediate objective is to understand the distribution of amiloride- and gurmarin-sensitive transduction mechanisms across taste receptor cells (TRCs) located in both the fungiform and palatal epithelia. Toward achievement of these aims, an experimental paradigm was chosen that allows transmembrane currents to be patch recorded from the basolateral membrane of individual TRCs in situ following taste stimulation that is restricted to the mucosal surface. Thus, this procedure permits one to chemically stimulate TRCs in a manner that is representative of how these cells would naturally interact with taste stimuli, which provides an unbiased method for measuring the tuning profiles of TRCs. Moreover, this preparation is ideally suited for determining the influence of both amiloride and gurmarin on the gustatory sensitivities of individual TRCs. When coupled with what is known regarding the effects of these substances on higher-order neuronal elements, data obtained from the proposed experiments may logically reveal the existence of specific synaptic links between particular types of TRCs and nerve fibers that express common sensitivity shifts following specific gustatory input blockage.