OLFACTORY AXON REGULATE OLFACTORY BULB CYTOGENESIS

描述(申请人提供)：神经发生和神经发育的协调 发展中的差异化对形成精准 神经回路。最近的研究提供了轴突的证据 与靶组织的相互作用可以调节神经发生。的具体目标 这个应用程序被设计用来检查嗅觉轴突 与发育中的端脑相互作用调节神经发生 嗅球原基，从鼻端发育而来。 端脑。我们将使用体外实验方法并结合这些方法 细胞周期动力学分析。主要的实验目标是：1) 观察早期嗅觉轴突在调节细胞周期动力学中的作用 在嗅球原基中；2)观察早期嗅觉 轴突在决定嗅球神经元是否 原基成为二尖瓣细胞；以及3)决定先驱嗅觉 轴突调节嗅觉形态发生的能力是独一无二的。 灯泡。这项工作的长期目标是阐明外在因素的作用 中枢神经系统神经产生调节机制中的信号，重点是 嗅球。我们预计，通过这项研究获得的证据将 深入了解中枢神经系统细胞的细胞周期是如何调控的。这个 确定细胞发生过程中的关键调控步骤将有助于 进一步分析参与这一过程的分子参与者。

DESCRIPTION (provided by applicant): The coordination of neurogenesis and differentiation in development is important for the formation of precise neuronal circuitry. Recent studies have provided evidence that axonal interaction with target tissue can regulate neurogenesis. The specific aims of this application are designed to examine the mechanisms by which olfactory axons interact with the developing telencephalon to regulate neurogenesis in the olfactory bulb primordium that develops from the rostral end of the telencephalon. We will use in vitro experimental approaches and combine these with cell cycle kinetics analysis. The major experimental goals are to: 1) examine the role of the early olfactory axons in regulating cell cycle kinetics in the olfactory bulb primordium; 2) investigate whether the early olfactory axons play a role in determining whether neurons in the olfactory bulb primordium become mitral cells; and 3) determine whether the pioneer olfactory axons are unique in their ability to regulate the morphogenesis of the olfactory bulb. The long-term goals of this work are to elucidate the roles of extrinsic signals in regulating mechanisms of neural production in CNS, with a focus on the olfactory bulb. We expect that evidence obtained through this study will provide insights into how the cell cycles of CNS cells are regulated. The determination of critical regulatory steps during cytogenesis will facilitate further analysis of the molecular players involved in this process.