ESTABLISHMENT OF A SPF RHESUS MACAQUE COLONY

该项目的目标是提高印度的能力，培育出对B型疱疹病毒、SIV、SRV和STLV-1无特定病原体(SPF)的印度源恒河猴(Macaca Mulatta)。拟建的殖民地将生产出高质量的具有基因特征的动物，供总部设在西南地区灵长类动物研究中心(SRPRC)的NIH受赠者以及美国西南部地区的其他研究机构用于艾滋病相关研究。新的SRPRC殖民地的大部分创始股票将通过将美国空军现有的SPF殖民地捐赠给灵长类中心而获得。美国空军殖民地目前位于距离SRPRC 20分钟车程的一流设施中，这些设施可以在本项目期间租赁给SRPRC。共有215只动物(120只雌性繁殖者，15只雄性繁育者，80只幼崽)将组成最初的SPF群体。拟议的生产和收购时间表将使种群在4年内翻一番，达到430只动物的稳定状态。这一目标群体规模将允许从04年开始每年收获70只动物，以满足艾滋病相关研究的关键需求。此外，在最初的三年中，每年都会收获30到50只动物进行研究。该群体产生的SPF猴子将具有良好的血清学和遗传学特征。将定期对阳性动物进行血清学检测和移走，以确保保持SPF状态。SPF群体的所有成员都将接受15个微卫星标记的基因分型，这些标记将用于评估母子关系和亲子关系，并对群体进行基因特征描述。这些详细的遗传信息将用于积极的遗传管理计划，旨在最大限度地减少近亲繁殖，最大限度地增加遗传变异，并确保群体具有完全的血统。每只动物的Mamu-AO1状态将被确定，并将对一部分群体采用选择性育种策略，以生产携带该MHC等位基因的动物。将使用人口管理战略，通过制定最佳的生产和收获时间表，确保种群的长期生存能力。在SRPRC的新设施建成后，将允许在5年授权期结束时在该中心提供殖民地住房。

The goal of this project is to increase the nation's capacity to produce Indian-origin rhesus monkeys (Macaca mulatta) that are specific pathogen-free (SPF) for herpes B virus, SIV, SRV, and STLV-1. The proposed colony will produce high quality genetically characterized animals for use in AIDS-related research by NIH grantees based at the Southwest Regional Primate Research Center (SRPRC) and at other research institutions in the southwestern region of the United States. Most of the founding stock of the new SRPRC colony will be obtained through donation of an existing SPF colony from the U.S. Air Force to the Primate Center. The U.S. Air Force colony is currently located a 20 minute drive from the SRPRC in excellent facilities that are available to be leased to the SRPRC for the duration of this project. A total of 215 animals (120 breeder females, 15 breeder males, and 80 juveniles) will comprise the initial SPF colony. The proposed production and acquisition schedule will result in doubling of the colony within 4 years to achieve a steady state of 430 animals. This target colony size will allow an annual harvesting of 70 animals per year beginning in Year 04 to fulfill critical needs in AIDS-related research. In addition, between 30 and 50 animals will be harvested for research during each of the first 3 years. The SPF monkeys produced by the colony will be well characterized serologically and genetically. Serological testing and removal of positive animals will be done on a routine basis to assure maintenance of SPF status. All members of the SPF colony will be genotyped for 15 microsatellite markers which will be used to assess maternity and paternity, and to genetically characterize the colony. This detailed genetic information will be used in an active genetic management program designed to minimize inbreeding, to maximize genetic variability, and to ensure that the colony is fully pedigreed. The Mamu-A O1 status of each animal will be determined, and a selective breeding strategy will be employed with a portion of the colony to produce animals which carry this MHC allele. Demographic management strategies will be used to ensure the long-term viability of the colony through development of optimal production and harvesting schedules. Completion of new facilities at the SRPRC will allow housing of the colony at the Center by the conclusion of the 5-year grant period.