CELL ADHESION FUNCTION IN THE VISUAL SYSTEM

描述(申请人提供)：多名钙粘素家族成员 在神经系统中表达。钙粘附素处于重要地位 生长激素迁移、丛生、散结和靶向的调节 发育过程中的细胞识别。各种研究都证实了 钙粘附素是发育过程中形态调节所必需的，并且 钙粘附素先前被证明调节视网膜顶盖的投射。至 了解钙粘附素介导的细胞间黏附如何控制分化 以及眼睛发育过程中的形态调节运动，我们将分离钙粘附素 细胞黏附分子在发育中的视觉系统和 确定钙粘附素的空间和时间表达模式 特别注意视觉系统的形态发生。斑马鱼模型 系统将用于研究钙粘附素的功能和调节 实验操作和遗传操作的简便性提供的优势 对发育途径的操纵。建议进行实验以检验 钙粘蛋白细胞黏附在发育中的视觉系统中的作用。钙粘附素 假设为各种不同的接线提供组合代码 神经系统中的回路。视网膜内的连通性，视网膜脱离 钙粘附素的黏附可能控制着投射和分离结构的投射。 对发育过程中钙粘附素表达的详细检查应该提供 对建立与视觉的连接的流程有重要的洞察力 系统。最后，我们建议对钙粘附素的表达进行实验操作 和功能，然后检查对神经元回路的影响 视觉系统，特别是形成视神经的视网膜顶盖连接 视顶盖的视网膜定位图。

DESCRIPTION (provided by applicant): Numerous cadherin family members are expressed in the nervous system. Cadherins are well positioned to be important regulators of growth cone migration, fasciculation, defasciculation and target cell recognition during development. Various studies have established that cadherins are necessary for morphoregulatory processes during development, and cadherins were previously shown to regulate retinotectal projection. To understand how cadherin-mediated cell-cell adhesion controls differentiation and morphoregulatory movements during eye development, we will isolate cadherin cell adhesion molecules that are expressed in the developing visual system and determine spatial and temporal expression patterns for cadherins with particular attention to morphogenesis of the visual system. The zebrafish model system will be used to study cadherin function and regulation due to the advantages provided by the ease of experimental manipulation and genetic manipulation of developmental pathways. Experiments are proposed to examine functions for cadherin cell adhesion in the developing visual system. Cadherins are hypothesized to provide a combinatorial code for the wiring of various circuits in the nervous system. Connectivity within retina, retinofugal projections and tectofugal projections may be controlled by cadherin adhesion. Detailed examination of cadherin expression during development should provide significant insight into processes that establish connectivity with the visual system. Finally, we proposed to experimentally manipulate cadherin expression and function, and then examine the consequences on neuronal circuits in the visual system, particularly the retinotectal connections that form the retinotopic map in the optic tectum.