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STRUCTURE/FUNCTION OF VINCULIN IN FOCAL ADHESIONS

STRUCTURE/FUNCTION OF VINCULIN IN FOCAL ADHESIONS
粘着斑中纽蛋白的结构/功能
批准号：
6526121
负责人：
ROBERT Colin LIDDINGTON
金额：
$37.51万
依托单位：
SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE
依托单位国家：
美国
项目类别：
财政年份：
1999
资助国家：
美国
项目状态：
已结题
起止时间：
1999-08-01 至 2003-07-31
来源：
https://reporter.nih.gov/project-details/6526121
关键词：
X ray crystallography animal tissue binding sites biological signal transduction cell adhesion focal adhesion kinase physical model protein binding protein folding protein protein interaction protein structure function site directed mutagenesis transcription factor vinculin

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中文摘要
翻译
描述：对粘着斑蛋白的结构进行了分析。 提出了利用X射线晶体学和核磁共振相结合， 为了确定全长黏着斑蛋白以及分离的结构域的原子模型， 然后确定与结合蛋白片段的复合物的结构。 这些数据将用于绘制结合位点，通过参考 完整分子的结构，也将表明如何调节结合。 利用这些信息，将产生缺乏特定功能的突变体 并在体内评估它们拯救黏着斑蛋白缺失细胞的能力。
英文摘要
DESCRIPTION: A structural analysis of the focal contact protein vinculin is proposed. Using a combination of X-ray crystallography and NMR, it is proposed to determine atomic models of full-length vinculin as well as isolated domains, and then determine the structures of complexes with binding-protein fragments. These data will be used to map out binding sites which, by reference to the structure of the intact molecule, will also suggest how binding is regulated. Using this information, mutants lacking specific functions will be generated and their ability to rescue vinculin null cells assessed in vivo.
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会议论文
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