HCG LH/CG RECEPTOR BINDING AND ACTIVATION

描述：（改编自申请人的摘要） 项目是阐明结构性决定因素， hCG与其受体的特异性高亲和力结合及其作用机制 配体介导的受体活化。 有两个主要目标，即1） 描述激素和受体结合决定簇和2）阐明 受体激活和跨膜信号传导的机制。 α亚基、hCG-β和LH/CG受体的定点突变 将被用来检验假设，即离散区域的hCG和 受体负责高亲和力结合。 调查将 利用酶-受体多肽融合技术构建了一个 推测的小促性腺激素 将利用受体诱变研究 为了检验跨膜螺旋6和7含有氨基酸的假设， 在信号传导中重要但在配体结合中不重要的残基。

DESCRIPTION: (adapted from the applicant's abstract) The objective of this project is the elucidation of the structural determinants leading to specific high-affinity binding of hCG to its receptor and the mechanism of ligand-mediated receptor activation. There are two major aims, namely 1) Delineation of hormone and receptor binding determinants and 2) Elucidating the mechanism of receptor activation and transmembrane signaling. Site-directed mutagenesis of alpha subunit, hCG-beta, and LH/CG receptor will be used to test the hypothesis that discrete regions of hCG and of receptor are responsible for high-affinity binding. The investigations will exploit a hormone-receptor polypeptide fusion technique to construct a putative mini-gonadotropin. Receptor mutagenesis studies will be utilized to test the hypothesis that transmembrane helices 6 and 7 contain amino acid residues that are important in signaling but not ligand binding.