CORE--MOLECULAR BIOLOGY

描述：分子生物学设施核心的总体目标是 为中心研究人员提供先进的 分子技术这一目标已经并将通过 以下具体目标：1）提供常规和专门的分子 生物服务，目前包括DNA分离，PCR，亚克隆，DNA 测序、基因分型和突变检测。2)提供教化和 协助使用成像采集和分析仪器， 序列数据分析和序列数据库检索，以及提供 高通量测序、DNA片段分析和 机器人3)为各种分子生物学提供专业知识和咨询 生物学方法4)确定、开发和实施新技术， 满足中心研究人员的需求。该设施核心已被一个 在当前的资助期内，中心成员的数量。通过 作为中心补助金的补充，他们能够从手册升级到 通过购买ABI 377自动化设备进行测序操作 测序仪他们还扩大了基于PCR的基因分型服务。新 已经获得了用于序列分析的计算机工作站， 成像仪器已被添加到核心，包括柯达图像 Station 440 CF，分子动力学荧光成像仪SI和分子动力学荧光成像仪SI 风暴820磷光成像仪。最近，这一核心已进入扩大和 新装修的空间，提供了一个成像实验室，湿 实验室和高通量/自动化实验室。未来方向 包括扩大自动化和基因分型服务，以及工作 与拟议的功能基因组学设施核心密切合作， 基因表达服务和DNA测序。

Description: The overall goal of the Molecular Biology Facility Core is to provide Center investigators access to sophisticated and state of the art molecular techniques. This goal has and will be accomplished through the following specific aims: 1) Provide both routine and specialized molecular biology services, currently including DNA isolation, PCR, subcloning, DNA sequencing, genotyping, and mutation detection. 2) Provide instruction and assistance in the use of imaging acquisition and analysis instruments, sequence data analysis, and sequence database searching, as well as provide supervised access to high throughput sequencing, DNA fragment analysis and robotics. 3) Provide expertise and consultation for a variety of molecular biology methodologies. 4) Identify, develop and implement new technologies to meet the needs of Center investigators. This Facility Core has been used by a number of Center members during the current funding period. Through a supplement to the Center grant, they were able to upgrade from a manual sequencing operation to an automated facility with the purchase of an ABI 377 sequencer. They also have expanded their PCR-based genotyping services. New computer workstations have been obtained for sequence analysis and several new imaging instruments have been added to the core, including a Kodak Image Station 440CF, a Molecular Dynamics FluorImager SI, and a Molecular Dynamics Storm 820 Phosphoimager. Most recently, this core has moved into expanded and newly renovated space that provides for an imaging laboratory, a wet laboratory, and a high throughput/automation laboratory. Future directions include expanded automation and genotyping services, as well as working closely with the proposed Functional Genomics Facility Core in the development of gene expression services and DNA sequencing.