Biomarkers of Oxidative Stress in Look AHEAD

描述（由申请人提供）： 超重和糖尿病会增加心血管疾病的风险， 造成这种影响的机制仍有待澄清。氧化 低密度脂蛋白（LDL）被认为是动脉粥样硬化的原因。 糖尿病可能会增加氧化应激，这可能有助于增加 糖尿病患者患心血管疾病的风险。热量限制和 开始运动可能会在短期内增加氧化应激， 从而不利地影响心血管疾病。从长远来看，代谢 适应可以减少氧化应激。这种适应，与 脂蛋白浓度、组成和特性的有益变化， 可能允许表达减肥对心血管疾病的有益作用， 端点。然而，体重减轻对脂蛋白测量的影响 氧化/氧化应激和抗氧化状态的超重糖尿病患者， 从未被调查过。这项研究的目的是确定如何减肥 通过强化生活方式干预，包括饮食和运动改变， 超重者脂蛋白氧化/氧化应激和抗氧化状态 糖尿病患者。该项目的主要目的是确定 强化生活方式干预对脂蛋白氧化作用影响 通过氧化低密度脂蛋白自身抗体评估。其他 氧化应激的补充指标和评估的抗氧化状态 通过亲脂性抗氧化剂的循环浓度， 生活方式干预和社区护理（控制）之间的武器看 AHEAD研究。这种比较将在干预后早期进行， 以及稍后预期代谢适应时。通过对比分组 根据对饮食或运动的不同依从性定义，按性别、绝经期 地位，种族和年龄，次要目标将考虑是否改变， 脂蛋白氧化/氧化应激和抗氧化状态之间存在差异 这样的亚组。其他目标将考虑脂蛋白的差异是否 上述亚组中的氧化/氧化应激可以通过以下方式解释： 脂蛋白浓度、组成或特性的差异， 碳水化合物代谢或抗氧化状态。这项研究的结果将 为心血管疾病的差异提供了潜在的机制解释， 干预组和社区护理组之间的终点。

DESCRIPTION (provided by applicant): Overweight and diabetes increase risk of cardiovascular disease but the mechanism(s) that account for this effect remain to be clarified. Oxidation of low-density lipoprotein (LDL) is thought to contribute to atherosclerosis. Diabetes may increase oxidative stress, and this may contribute to increased risk of cardiovascular disease in diabetics. Caloric restriction and the initiation of exercise may increase oxidative stress in the short term and may thus adversely affect cardiovascular disease. Over the long term, metabolic adaptation may reduce oxidative stress. Such adaptation, together with beneficial changes in lipoprotein concentration, composition, and character, may allow expression of beneficial effects of weight loss on cardiovascular endpoints. However, the influence of weight loss on measures of lipoprotein oxidation/oxidative stress and antioxidant status in overweight diabetics has never been investigated. The goal of this study is to determine how weight loss achieved by intensive lifestyle intervention including diet and exercise alters lipoprotein oxidation/oxidative stress and antioxidant status in overweight diabetic individuals. The primary aim of this project is to determine the influence of intensive lifestyle intervention on lipoprotein oxidation as assessed by autoantibodies to oxidized low-density lipoprotein. Other complementary indices of oxidative stress, and antioxidant status as assessed by circulating concentrations of lipophilic antioxidants, will be compared between lifestyle intervention and community care (control) arms of the Look AHEAD study. Such comparisons will be performed both early after intervention and later when metabolic adaptation is expected. By contrasting subgroups defined by differential compliance to diet or exercise, by gender, menopausal status, ethnicity, and age, secondary aims will consider whether changes in lipoprotein oxidation/oxidative stress and antioxidant status differ between such subgroups. Other aims will consider whether differences in lipoprotein oxidation/oxidative stress among the above subgroups can be accounted for by differences in lipoprotein concentration, composition, or character, carbohydrate metabolism, or antioxidant status. The results of this study will provide a potential mechanistic explanation for differences in cardiovascular endpoints between intervention and community care groups.