Wide field imaging at depth for the biomedical sciences

光片显微镜(LSM)是一种快速、大视场、低光毒性成像的有效方法。它的最终极限是光学穿透到样品中。现有的克服这一挑战的方法包括从多个侧面照亮样品，使用化学清除方法或复杂的像差校正。虽然有效，但一个挑战是为生物医学研究人员和病理学的紧急使用领域开发简便的方法。最近在高分辨率LSM方面的进展，包括我们团队的进展，利用了先进的传播不变模式，特别是贝塞尔、艾里和‘格子’模式，以获得高分辨率的大视场。这可以在100微米的视场内达到0.000001米的分辨率，但我们如何才能获得优异的时空分辨率(例如，100秒赫兹，视场可达毫米，亚衍射成像)？为此，我们也可以使用超分辨率成像。这将包括结构照明显微镜以及其他算法。这个博士项目将探索光片和超分辨率成像的适当组合，用于生物医学研究。具体研究将包括结肠癌、肺癌和肾脏疾病的组织病理学应用，并对适当的组织样本进行成像。

Light-sheet microscopy (LSM) is a powerful approach for fast wide field, low phototoxicity imaging. Its ultimate limit is optical penetration into the specimen. Existing approaches to overcome this challenge include illuminating the sample from multiple sides, the use of chemical clearing methods or sophisticated aberration correction. Though effective, a challenge is to develop facile methods for biomedical researchers and the emergent area of use in pathology. Recent advances, including those by our team, in high-resolution LSM have exploited advanced propagation-invariant modes of light, in particular Bessel, Airy and 'lattice' modes, for wide field of view with high resolution. This can achieve 0.000001 m resolution over 100s of micron field of view, but how may we obtain excellent spatiotemporal resolution (e.g. 100s Hz, FOV up to mm, sub diffractive imaging)? For this we can use super-resolution imaging as well. This will include structured illumination microscopy as well as other algorithmsThis PhD project will explore appropriate combinations of light sheet and super-resolved imaging for studies in biomedicine. Particular studies will include applications for histopathology for colon cancer, lung cancer and renal disease with imaging on appropriate tissue samples.