Histidine-Rich Glycoprotein: Characterization and Clinical Significance Studies

富组氨酸糖蛋白(HRGP)，一种血浆 具有抗肝素和抗纤溶活性的蛋白质已经被 与血栓形成有关的有三个家族。因此，它可能 促进高凝状态。以下是我们的目标 在实验室中实现的：使用 钴-琼脂糖凝胶的组合及其应用研究 用酶联免疫吸附测定法测定其含量。我们有 确认了HRGP的抗肝素活性。此外，我们还有 证明HRGP能与活化的人血小板结合。这个 几个二价阳离子增加了结合量，但不是 钙或镁。它还通过以下方式得到显著增强 纤溶酶原，尽管HRGP没有相互作用 增加纤溶酶原与血小板的结合。这些观察结果 提示血小板结合的纤溶酶原可能作为结合部位 对于HRGP来说，虽然似乎还有其他网站。

Histidine-rich glycoprotein (HRGP), a plasma protein with antiheparin and antifibrinolytic activity, has been associated with thrombophilia in three families. Therefore, it may promote hypercoagulability. The following objectives have been achieved in the laboratory: purification of the HRGP, using a combination of cobalt-sepharose, and development of an enzyme-linked immunosorbent assay for its quantitation. We have confirmed HRGP's antiheparin activity.In addition, we have demonstrated that HRGP binds to activated human platelets. The amount of binding is increased by several divalent cations, but not calcium or magnesium. It is also enhanced significantly by plasminogen, although HRGP does not have the reciprocal effect of increasing plasminogen binding to platelets. These observations suggest that platelet-bound plasminogen may serve as a binding site for HRGP, although there appear to be other sites as well.