DOPAMINE TRANSPORTER/MU OPIATE RECEPTOR--CELLULAR AND SUBCELLULAR LOCALIZATIONS

多巴胺转运体(DAT)和MU阿片剂 受体(MOR)已被确定为主要的大脑受体 与奖励和愉悦特性相关的网站 可卡因和吗啡。蜂窝和亚蜂窝定位数据是 重要的是有助于阐明这些的功能属性 重要的大脑分子。在本财年，有关分发 大鼠脑内的DAT和MOR蛋白已基本完成。 对转运蛋白的超微结构研究表明，大多数 DAT和MOR在细胞内和细胞内呈阳性反应 质膜定位不容易与 传统的突触特化。这些研究实质上 添加有关这些组件的详细蜂窝定位的信息 可卡因和吗啡奖励的重要地点。他们强调 突触外移除多巴胺的可能性提供了一个 DAT的主要功能。

The dopamine transporter (DAT) and mu opiate receptor (MOR) have been identified as the principal brain receptor sites correlated with the rewarding and euphoric properties of cocaine and morphine. Cellular and subcellular localization data are important to help to elucidate the functional properties of these important brain molecules. In this FY, reports of the distribution of the DAT and MOR proteins in rat brains were largely completed. Ultrastructural studies of the transporter revealed that most of the DAT and MOR immunoreactivity was found in intracellular and plasma membrane localizations not readily associated with conventional synaptic specializations. These studies substantially add to information on the detailed cellular localizations of these important sites for cocaine and morphine reward. They underscore the likelihood that extrasynaptic removal of dopamine provides a principal function of DAT.