Using a combined approach of molecular microbiology, genomic mining and phylogenomics to discover and activate cryptic biosynthetic gene clusters in S

放线菌是土壤细菌的一种，是新型抗菌剂的丰富来源。链霉菌是放线杆菌中最大的细菌类群，具有许多已发现、未发现和推定的隐蔽基因簇。深度测序技术、分子克隆技术和数据挖掘技术的进步使得通过分析和操纵生物合成操纵子中的调控因子来预测新的基因途径成为可能。该项目将利用一种组合的方法来瞄准各种土壤，以恢复完整的生物合成基因簇，并刺激其隐蔽产物的表达。这样的过程有望导致发现新的天然化合物，如抗生素和抗癌药物。

Actinobacteria are a type of soil bacteria and are a rich source of novel antimicrobials. Streptomyces is the largest group of bacteria in Actinobacteria and possess a number of discovered, undiscovered and putative cryptic gene clusters. Advances in deep sequencing technology, molecular cloning techniques and data mining has allowed the prediction of novel gene pathways through analysis and manipulation of regulatory factors in biosynthetic operons. This project will utilise a combined approach to target various soils to recover intact biosynthetic gene clusters and stimulate the expression of their cryptic products. Such processes will hopefully result in the discovery of novel natural compounds such as antibiotics and anticancer drugs.