Nanoscale Devices on DNA Functionalized Semiconductors

描述(由申请者提供)：本研究项目的目标是 基于纳米颗粒的DNA检测方案的接口半导体电路 采用浸渍笔纳米光刻(DPN)技术。该项目将得到发展 优化半导体上固定化寡核苷酸所需的化学条件 以与DPN工艺兼容的方式加工表面。修改 原子力显微镜/DPN针尖提高寡核苷酸效率 将继续进行油墨沉积，并将使用DPN制造的基板 组装和表征DNA功能化纳米颗粒的图案。这个 项目将开发和研究基于 纳米粒子的电学和近场光学性质 表面结合的DNA，并将开发原理证明演示 集成的纳米级生物传感器电路。这些计划不仅提供了 承诺提高灵敏度和选择性 基于荧光团的检测方法，但它们的发展也涉及 生物系统中纳米科学的基本问题，包括 扩散、电荷传输和能量转移。

DESCRIPTION (provided by applicant): The goal of this research program is to interface semiconductor circuitry with nanoparticle-based DNA detection schemes using the technique of Dip Pen Nanolithography (DPN). The project will develop optimize the chemistry needed to immobilize oligonucleotides on semiconductor surfaces in a manner compatible with the DPN process. Modifications of the atomic force microscope/DPN tip to enhance the efficiency of oligonucleotide ink deposition will be pursued and the DPN fabricated substrates will be used to assemble and characterize patterns of DNA functionalized nanoparticles. The project will develop and study new single-label detection schemes based on the electrical and near-field-optical properties of nanoparticles with surface-bound DNA, and will develop proof-of-principle demonstrations of integrated nanoscale biosensor circuitry. Not only do these schemes offer the promise of enhanced sensitivity and selectivity as compared to fluorophore-based detection methods, but their development also addresses fundamental issues of nanoscience in biological systems including those of diffusion, charge transport and energy transfer.