Development of the Precerebellar System

描述(申请人提供)：对小脑功能至关重要的是一组 五个脑干神经元组件(核)统称为 小脑前部系统。这些核团负责传递和处理 从大脑皮层和脊髓流向大脑 小脑。小脑前神经元由背侧神经上皮细胞产生 (菱形嘴唇)位于延髓和脑桥的腹侧 脑干。为了到达它们的最终位置，这些神经元必须长时间迁移 穿过几个脑区的距离，密密麻麻的 突触连接的神经丛。因此，解决发展的研究 这种关键的、但很少被研究的神经复合体应该进一步阐明 成熟大脑中潜在的细胞迁移机制以及对 有助于我们理解小脑功能的一个关键方面。使用克隆 基因表达的分析、序列分析和基于重组酶的命运 作图和诱变技术，拟议的项目将集中在 小脑前神经元同一性在脑内建立的机制 菱形嘴唇。

DESCRIPTION (provided by applicant): Critical to cerebellar function is a set of five brainstem neuron assemblies (nuclei) collectively referred to as the precerebellar system. These nuclei are responsible for relaying and processing information that flows from the cerebral cortex and spinal cord to the cerebellum. Precerebellar neurons are generated from dorsal neuroepithelium (the rhombic lip) yet settle in ventral aspects of the medulla and pontine brainstem. To reach their final positions, these neurons must migrate long distances across several brain subdivisions filled with densely packed, synaptically-connected neuropile. Thus, research addressing the development of this crucial, yet rarely studied, neural complex should further elucidate mechanisms underlying cell migration in the mature brain, as well as contribute to our understanding of a critical aspect of cerebellar function. Using clonal analysis, serial analysis of gene expression, and recombinase based fate mapping and mutagenesis techniques, the proposed project will focus on the mechanisms by which precerebellar neuronal identity is established within the rhombic lip.