A Dual Electrospray/Photoionization Source

提出了一种将电喷雾（ES）装置与大气压光电离（PI）源集成的方法.我们将采用一种专有的方法来产生带电液滴，这些液滴将经历电荷排斥以形成挥发的分析物而不产生ES正离子。正离子光谱将仅由PI引起。通过这种方式，PI的益处可以扩展到非挥发性和热不稳定的化合物。PI已被证明是用于分析各种化合物的通用电离源，在许多情况下，化合物无法通过ES电离（ESI）或大气压化学电离（A PCI）检测到。PI特别适用于药物样化合物，并且对影响ESI和APCI质谱的电荷竞争和离子抑制不太敏感。这是一个重新提交的提案，其中包含了新的结果，大大提高了成功的可能性。我们相信，如果SBIR提供种子资金，以测试第一阶段的可行性和解决第二阶段的研发问题，ES/PI来源将立即商业化。ES/PI源还可以作为ES电离源操作，从而为大分子提供双重ESI和PI能力。后一种战略将导致制药业迅速接受和广泛使用。拟定商业应用：制药行业对分子分析技术在速度、定量和通用性方面的要求越来越高。双ESI/PI源的开发将大大扩展能力，并将被广泛接受作为增强的ESI源使用。

We propose a method to integrate an electrospray (ES) device to an atmospheric pressure photoionization (P I) source. We will employ a proprietary method to generate charged droplets that will undergo charge-repulsion to form volatilized analyte without producing ES positive ions. The positive ion spectrum will be due to PI only. By this means, the benefits of PI can be extended to non-volatile and thermally- labile compounds. PI has proven to be a general purpose ionization source for analyzing a wide variety of compounds, in many cases compounds not detectable by ES ionization (ESI) or atmospheric pressure chemical ionization (A PCI). PI is particularly well suited for drug-like compounds, and is much less susceptible to competition-for- charge and ion-suppression that affects ESI and APCI mass spectra. This proposal is a resubmission that contains new results that greatly raise the probability of success. We are confident that if provided SBIR seed money to test the feasibility in Phase I and solve the R&D issues in Phase II, that the ES/PI source would be immediately commercializable. The ES/PI source could also operate as an ES ionization source thereby providing dual ESI and PI capabilities for large molecules. The latter strategy would lead to rapid acceptance and widespread use in the pharmaceutical industry. PROPOSED COMMERCIAL APPLICATION: The pharmaceutical industry places increasingly greater demands on molecular analysis technology in terms of speed, quantitation, and generality. Development of a dual ESI/PI source would greatly expand capabilities and would be widely accepted into use as an enhanced ESI source.