Core--Recombinant viral gene transfer

(改编自申请人的摘要)核心的目的是提供 用于高效生成和交付的资源和专业知识 重组表达病毒(腺病毒和逆转录病毒)的胚胎 含有项目调查人员感兴趣的基因。(摘要结束) 这个核心提供了关键的腺病毒和逆转录病毒支持 本提案中的项目。大多数项目建议要么使用血统 使用腺病毒或逆转录病毒追踪或表达某些基因 转染小鼠或鸡胚胎培养物。这个核心是中心 在不同项目中概述的实验。 首席调查员和合作调查员对腺病毒有经验。 并提出了逆转录病毒技术。他们的方法很直截了当 而且是适当的。他们将维持这些实验所需的细胞系 并产生实验所需的高滴度病毒库存。他们 建议将核心实验室提供给调查人员，以便他们 学习并提供这项服务。提供直接感染的计划 培养中的整个胚胎(小鼠和鸡)的比例是合理的，并且 调查人员对这类技术有经验。总体上没有 提出的方法存在明显的困难。 建议的实验类型现在相当常规，将是有用的 用于各个项目中概述的各种实验。一种能力 在整个胚胎培养中感染特定组织是相当创新的， 尽管它已经被其他调查人员成功地使用。不过， 这不是一项常见的技能，而这一核心肯定会是一种优势 拥有这种能力。 首席调查员和联合调查员都有很好的经验，因为他们 已经利用逆转录病毒和Adno病毒技术进行了演示。他们有 为了其他目的成功地完成了这些类型的实验，并且应该 在执行所列任务方面没有任何困难。库巴拉克医生已经 在小鼠胚胎培养方面的经验，这肯定是一种财富 该计划的项目拨款。 核心的环境很强大，他们似乎已经拥有了所有 概述了提供核心服务所需的设备和技术。 环境方面没有任何问题。 这是该计划项目的一个非常重要的核心，看起来 设计得当，没有任何重大缺陷。核心有很多优点 对于大多数项目来说，这将是一个关键问题。有空的医生。 作为一名顾问，Mikawa进一步增强了这个核心的能力 解决问题并使其有效。

(Adapted from the Applicant's Abstract) The purpose of the core is to provide the resources and expertise for the efficient generation and delivery into embryos of recombinant expression viruses (adenovirus and retroviruses) containing genes of interest to the project investigators. (End of Abstract) This core provides the critical adeno-viral and retro-viral support for the projects in this proposal. Most of the projects propose to either use lineage tracing or expression of certain genes utilizing either adeno-viral or retro-viral transfection into mouse or chick embryo cultures. This core is central to the experiments outlined in the various projects. The principal investigator and co-investigators have experience in the adeno-viral and retro-viral techniques proposed. Their approach is straight-forward and appropriate. They will maintain cell lines necessary for these experiments and generate the high titer viral stocks necessary for the experiments. They propose to make the core laboratory available to the investigators for them to learn as well as provide this service. The plans to provide direct infection of whole embryos in culture (mouse and chicken) are reasonable, and the investigators have experience with such techniques. There are overall no glaring difficulties with the proposed approach. The types of experiments proposed are now rather routine and will be useful for the various experiments outlined in the individual projects. The ability to infect specific tissues in whole embryo cultures is rather innovative, although it has been used successfully by other investigators. Nevertheless, it is not a common skill and it is certainly a strength that this core will have that ability. The prinicipal investigator and co-investigators have good experience, as they have demonstrated, utilizing retro-viral and adno-viral techniques. They have done these sorts of experiments for other purposes successfully, and should have no difficulty in performing the tasks outlined. Dr. Kubalak has had experience with mouse embryo culture and this will certainly be an asset to the program project grant. The environment for the core is strong and they appear to already have all the necessary equipment and technology to provide the core services outlined. There are no problems with the environment. This is a very important core for this program project and appears to be designed appropriately without any major flaws. The core has many strengths and will be a critical one for most of the projects. The availability of Dr. Mikawa as a consultant adds furthur strength to the ability of this core to troubleshoot and be effective.