CORE--TISSUE CULTURE AND MONOCLONAL ANTIBODY

描述：（申请人描述）组织培养/单克隆 抗体核心（TC/MB）在过去的12年里一直是一种有效的 UCCC研究人员获取专业细胞培养的资源 技术和生产单克隆抗体（MAbs）。细胞融合和 自2000年以来，已经提供了分泌MAb的杂交瘤细胞系的构建。 该设施的建立，并继续是一个主要职能。生产 来自常规旋转容器中杂交瘤大规模生长的MAb， 在中空纤维生物反应器中， 在过去的四年中，杂交瘤服务的增长率约为300%。 年杆状病毒载体的构建及重组质粒的表达 昆虫细胞中的蛋白质，在上次审查中作为一项服务建立， 也显著增长（200-300%），成为核心的主要功能。 技术合作/管理局核心组已开发并维持了一个150多个储存库， 癌细胞系，并为UCCC研究人员提供了 用于实验的培养物或来自大体积培养物的细胞团， 用于分离大分子或其它细胞因子的来源。1997年， 现场媒体供应中心成立， 制备的液体生长培养基、补充剂和血清， 价格，并节省了UCCC调查人员超过18万美元。除了 技术合作/管理局核心提供咨询/培训和质量管理， 控制程序。一项或多项技术合作/管理局核心服务的使用者 自上次审查TC/MB以来，有117名UCCC调查员。用户来自 10个项目中有9个，超过95%的项目进行了同行评审的校外研究 赠款。定制技术服务为超过100个国家提供了支持。 在过去的4年里，UCCC研究人员发表了180篇出版物。未来的目标 技术合作/管理咨询委员会核心部分的任务是继续提供现有的高质量服务， 扩大定制杆状病毒服务的能力， 增加最多的杂交瘤。为了扩大这些 服务，要求核心赠款预算每年小幅增加5.5%， 技术人员可以从3人增加到4人。此外，本发明还 第二年需要新设备。

Description: (Applicant's Description) The Tissue Culture/Monoclonal Antibody Core (TC/MB) has served for the past 12 years as an efficient resource for UCCC investigators to access specialized cell culture technologies and produce monoclonal antibodies (MAbs). Cell fusion and construction of MAb secreting hybridoma cell lines has been offered since the founding of the facility and continues to be a major function. Production of MAbs from large scale growth of hybridomas in conventional spinner vessels and in hollow-fiber bioreactors was established more recently and this component of hybridoma services has grown by approximately 300% over the past four years. Construction of baculovirus vectors and expression of recombinant proteins in insect cells, established as a service at the last review, has also grown significantly (200-300%) to become a major function of the Core. The TC/MB Core has developed and maintains a repository of more than 150 cancer cell lines in liquid nitrogen and provides UCCC investigators with seed cultures for experiments or cell pellets from large volume cultures as a source for isolation of macromolecules or other cellular factors. In 1997, an On-Site Media Supply Center was established that provides commercially prepared liquid growth medium, supplements and serum at substantially reduced prices and has saved UCCC investigators over $180,000. In addition to technical services, the TC/MB core provides consultation/training and quality control procedures. One or more of the TC/MB Core services has been used by 117 UCCC investigators since the last review of the TC/MB. The users are from 9 of 10 Programs and more than 95% hold peer-reviewed extramural research grants. The custom technological services have provided support for more than 180 publications of UCCC investigators over the last 4 years. The future goal of the TC/MB Core is to continue to provide existing quality services and expand the capacity of custom baculovirus services and large scale growth of hybridomas that have increased the most. To expand the capacity of these services, a small annual increase of 5.5% in Core grant budget is requested so that the technical staff can increase from 3 to 4 individuals. Additionally, new equipment is requested in the second year.