CORE--MOLECULAR GENETICS

描述：(改编自申请人的摘要)用于连锁和关联的基因型别是为了支持当前SCOR中的研究人员而进行的。总共运行了21个基因的41个不同的标记系统。本研究共获得6,647个基因分型。由于在整个赠款过程中不断有新的样本到达核心，并不是所有标记系统的所有样本都进行了分类，但已努力完成对关键标记系统的新样本的分类，这在最初的分析中产生了令人振奋的结果。分子遗传学核心目前正在完成对总共214个兄弟姐妹对样本中最后一组的基因分型和关联性研究，以及另外120个单一样本的基因分型，总共434个DNA样本。运行与候选基因相近的连锁标记以及候选基因内的单核苷酸多态(SNPs)。使用放射性/丙烯酰胺检测系统进行连锁标记。用质谱仪，Perkin-Elmer ABI Prism 7700的等位基因识别系统，或限制性内切酶消化，然后在琼脂糖凝胶上分辨来检测SNPs。

DESCRIPTION: (Adapted from the applicant's abstract) Genotypes for linkage and association were carried out in support of investigators in the current SCOR. A total of 41 separate marker systems from 21 genes were run. In all, 6,647 genotypes were obtained for this study. As new samples were arriving at the Core throughout the course of the grant, not all of the samples have been typed for all marker systems, however, an effort was made to complete typing on the new samples for key marker systems that yielded promising results in the original analysis. The Molecular Genetics core currently is completing the genotyping on the last group of a total of 214 sibling pair samples and for association studies and an additional 120 singleton samples for a total of 434 DNA samples. Linkage markers close to candidate genes as well as single nucleotide polymorphism (SNPs) within the candidate genes were run. Linkage markers were carried out using a radioactive/acrylamide detection system. SNPs were detected with either mass spectrometry, the allelic discrimination system of the Perkin-Elmer ABI Prism 7700, or restriction enzyme digestion followed by resolution on agarose gels.