CORE--BIOCHEMISTRY

描述：（改编自申请人的叙述）生化核心实验室以比在商业或医院实验室分析样品更有效和更具成本效益的方式进行常规生化分析。集中的生化核心设施还确保了分析结果的一致性，维护了完整的集中记录，并为每个分析定义了明确的质量控制指南。核心将(1)建立并确保样品收集、标签、储存和运输的统一方法；（二）为项目调查人员提供可靠、可重复、统一、及时的尿液、血浆和组织样本分析；(3)保存硬拷贝记录，并将所有生化和激素测量结果输入电子表格；(4)监督和维护项目调查员使用的核心共用设备，并对项目调查员实验室的人员进行正确使用设备的培训；(5)提供专业知识和设备，协助调查人员根据需要开发新的检测方法。该核心设施将提供并确保样品的统一采集、可靠的分析、记录的维护、共享设备的维护以及新检测方法的开发。在过去4年的项目中，从400多名患者和650只大鼠中收集和分析了26,568个样本。对人类样本进行了18种不同的分析，对大鼠样本进行了11种不同的分析。除了传统的生化测定（葡萄糖、脂质、肌酐、电解质等）外，还进行了放射免疫测定和高效液相色谱测定（儿茶酚胺、肾素、醛固酮、胰岛素、加压素）。在资助期间，还开发了新的检测方法，包括从人类样品中提取ANF的新方法，检查非裔美国人和法裔加拿大人血浆的冷冻活化率，以寻找血浆肾素活性的巨大差异，开发瘦素检测，尿白蛋白的新EIA，以及将cGMP和cAMP的RIA转换为更高通量的微滴板EIA。预计在今后的项目年中，将向实验室提供53,050个样品。这是这个项目的重要组成部分。它组织严密，装备精良。工作人员都很有能力，这将很好地为项目服务。

DESCRIPTION: (Adapted from the applicant's narrative) The Biochemical Core laboratory performs routine biochemical analyses in a more efficient and cost effective manner than having the samples analyzed in commercial or hospital based labs. The centralized Biochemical Core facility also ensures uniformity of assay results, maintains complete, centralized records and well defined quality control guidelines for each assay. The core will (1) establish and ensure uniform methods for the collection, labeling, storage, and transport of samples; (2) provide reliable, reproducible, uniform and timely analyses of urine, plasma and tissue samples for the project investigators; (3) maintain hard copy records and to enter the results for all biochemical and hormone measurements on electronic spreadsheet; (4) oversee and maintain shared use equipment in the core that is utilized by project investigators and train personnel of project investigator's laboratories in the proper use of the equipment; and (5) provide expertise and equipment to assist investigators in developing new assays as needed. (End of Abstract) This core facility will provide and ensure uniform collection of samples, reliable analyses, maintenance of records, maintenance of shared equipment, and development of new assays. In the past 4 years of the project, 26,568 samples have been collected and analyzed from over 400 patients and 650 rats. Human samples have been analyzed in 18 different assays while rats samples have been analyzed in 11 different assays. In addition to the traditional biochemical assays (glucose, lipids, creatinine, electrolytes, etc.), radioimmunoassays and HPLC measurements were made (catecholamines, renin, aldosterone, insulin, vasopressin). New assays were also developed during the funding period including a new extraction method for ANF from human samples, examination of the rate of cryoactivation of African American vs. French Canadian plasma to look for the large differences seen in plasma renin activities, development of a leptin assay, a new EIA for urinary albumin, and conversion of RIA's for cGMP and cAMP to higher throughput microtiter plate EIA's. It is anticipated that in the coming project years 53,050 samples will be presented to the laboratory. This is an important component of this program. It is extremely well organized and well equipped. The personnel are very capable, and it will serve the program well.