Core--Biochemical and Analytical Core

该计划项目赠款的生化核心实验室将提供一个整合的、高度专业化的、设备齐全的专业人员单位，能够根据项目调查人员的要求进行各种放射免疫分析、生化和基于高效液相的分析。可用的检测方法包括用高效液相色谱法测定一氧化氮合酶活性，用荧光高效液相色谱法测定EETs和20-HETE，用高效液相色谱法分析AA的代谢、磷脂酶活性，以及测定组织中cAMP、cGMP、IP3和DAG的浓度。中央生化核心设施将维护所有的分析设备，确保该计划中不同实验室之间的分析结果的一致性，并将维护完整的集中记录，并为每种分析提供明确定义的质量控制指南。将人员和设备集中在生化核心实验室将使实验室能够以更有效和更具成本效益的方式进行常规生化分析，而不是让调查人员在各自的实验室内进行这些分析。获得这个中心设施的分析专业知识和大量的设备对本计划的每个拟议项目都非常重要，建议研究中需要进行大量的生化和高效液相测定，因此支持我们使用这个设施是合理的。

The Biochemical Core Laboratory of this Program Project Grant will provide a consolidated, highly specialized, well equipped and professionally staffed unit capable of performing a variety of radioimmunoassay, biochemical and HPLC based analysis as required by project investigators. The available assays include measurements of NOS activity using a HPLC assay, fluorescent HPLC measurement of EETs and 20-HETE, HPLC analysis of the metabolism of AA, phospholipase activity and measurements of tissue concentrations of cAMP, cGMP, IP3 and DAG. The centralized Biochemical Core facility will maintain all of the analytical equipment, ensure uniformity of assay results between the different laboratories in this program and will maintain complete, centralized records and provide well-defined quality control guidelines for each assay. Centralizing the personnel and equipment in the Biochemical Core laboratory will allow the laboratory to perform routine biochemical analyses in a more efficient and cost-effective manner than if the investigators had setup to perform these analyses within their individual laboratories. Access to the analytical expertise and tremendous amount of equipment in this central facility is very important to each of the proposed projects of this Program and support for our use of this facility is justified by the large number of biochemical and HPLC determinations required in the proposed studies.