Roles of non-coding RNAs in translational control in response to environmental stress

真核生物基因组被广泛转录，产生许多不同的RNA，功能未知。基因表达在多个水平上响应于环境胁迫而被调节，包括在蛋白质合成水平上。一个未充分研究的领域是非蛋白编码RNA（ncRNA）如何在真核生物酵母酿酒酵母（Saccharomycescerevisiae）模型中对翻译控制做出贡献。该项目将解决这个问题。该项目采用了新的工作方式，因为它将涉及学生进行计算和实验室实验的混合，包括生成和分析高通量RNA测序数据集，以检查ncRNA与积极翻译核糖体的关联，然后通过实验研究它们对翻译控制的影响，以应对一系列环境压力。该项目将建立在监督团队的现有优势基础上，并利用各种实验室补充专业知识和资源，包括最近生成的一系列1502个分子条形码ncRNA基因缺失菌株，其中包括用于高通量功能分析的各种ncRNA。因此，该项目正在利用现代技术进行基本的机械分子生物学研究，并代表了支撑生物科学的世界级研究。

Eukaryotic genomes are extensively transcribed generating many different RNAs with no known function. Gene expression is regulated at multiple levels in response to environmental stresses, including at the level of protein synthesis. One under-researched area is how non-protein coding RNAs (ncRNAs) contribute to translational controls in the model eukaryote yeast Saccharomyces cerevisiae. The project will address this question. The project embraces new ways of working because It will involve the student undertaking a mix of computational and lab experiments including generating and analysing high-throughput RNA sequencing data sets to examine the association of ncRNAs with actively translating ribosomes and then studying experimentally their impact on translational controls in response to a range of environmental stresses. The project will build on existing strengths of the supervisory team and make use of various laboratory complementary expertise and resources including a recently generated series of 1502 molecular barcoded ncRNA gene deletion strains encompassing a wide range of ncRNAs for high-throughput functional analyses. Hence, the project is undertaking fundamental mechanistic molecular biology research while making use of modern technologies and represents World Class Research Underpinning the Biosciences.