Roles of meiotic-stage non-coding RNAs in maize anther development

减数分裂阶段非编码RNA在玉米花药发育中的作用

• 批准号: 2320971
• 负责人: Blake Meyers
• 金额: $ 100万
• 依托单位: Donald Danforth Plant Science Center
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2026-08-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2320971&HistoricalAwards=false
• 关键词: Roles; meiotic; stage; non; coding

Pollen production and regulation of plant fertility are essential to agriculture. Hybrid corn and other grains have significantly boosted world food production. This project will investigate the functions of RNAs that support pollen development in anthers (the organs that make pollen in flowering plants). The project will utilize plant genomics and developmental biology to determine the biological roles of small RNAs and the proteins that utilize them for anther functions. These RNAs are required for robust male fertility: in the absence of the small RNAs, at normal temperatures, development fails, yielding male sterility. Fertility can be rescued under lower temperatures, linking environmental conditions to the role of these small RNAs. The project focuses on maize because it is an optimal system for the proposed experiments; plus, male fertility is key to the production of hybrid corn seed. Outcomes of this project could include improved control of male fertility in grass crops. Broader impacts of the project include training of students in plant and RNA biology. The project will focus specifically on the class of 24-nt phased, secondary siRNAs, known as “24-nt phasiRNAs” that are highly enriched in meiotic-stage anthers. The project will characterize phenotypes and molecular genetic analysis of loss-of-function knock-outs including the Dicer-like5 (Dcl5) gene, for which the preliminary data demonstrate male sterility. The questions to be addressed by this project include which basic Helix-Loop-Helix transcription factors function to activate transcription of the 24-nt phasiRNAs? How does the core biogenesis protein DCL5 function, and what is the exact nature of the phenotype of sterility in its absence? Are there genetic modifiers of the dcl5 conditional phenotype in other backgrounds of maize? What are the key catalytic Argonaute proteins that load the 24-nt phasiRNAs for their function? And what are the target RNAs with which the phasiRNA-loaded Argonaute proteins interact?This award was co-funded by the Genetic Mechanisms Cluster in the Division of Molecular and Cellular Biosciences and the Plant Genome Research Program in the Division of Integrative Organismal Systems.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

花粉产生和植物生育能力的调节对于农业至关重要。混合玉米和其他谷物显着增强了世界粮食生产。该项目将研究支持其他人花粉发育的RNA功能（在开花植物中制造花粉的器官）。该项目将利用植物基因组学和发育生物学来确定小RNA的生物学作用以及将其用于其他功能的蛋白质。这些RNA是强大的男性生育所必需的：在没有小的RNA的情况下，在正常温度下，发育失败，产生男性不育。可以在较低的温度下挽救生育能力，将环境条件与这些小RNA的作用联系起来。该项目专注于玉米，因为它是提出的实验的最佳系统。另外，男性生育能力是杂化玉米种子产生的关键。该项目的结果可能包括改善草作物中男性生育能力的控制。该项目的更广泛影响包括对植物和RNA生物学的学生培训。该项目将专门集中于24-NT分阶段的次级sirnas的类别，即“ 24-nt Phasirnas”，它们在减数分裂阶段高度富集。该项目将表征表型和功能丧失敲除的分子遗传分析，包括DICER-LIKE5（DCL5）基因，初步数据证明了男性不育。该项目要解决的问题包括哪些基本的螺旋 - 环螺旋转录因子起作用以激活24-nt phasirnas的转录？核心生物发生蛋白DCL5如何发挥作用，在不存在的情况下，无菌表型的确切性质是什么？在玉米的其他背景中，是否有DCL5条件表型的遗传修饰符？为其功能加载24-nt phasirnas的关键催化argonaute蛋白是什么？与Phasirna负载的Argonaute蛋白相互作用的目标RNA是什么？该奖项是由遗传机制集群共同资助的分子和细胞生物科学部门的遗传机制，以及在综合有机体系统的植物基因组研究计划中，这一奖项通过评估NSF的法定委员会的范围来表达了综述的综述，这表明了这一奖项的综述，这表明了良好的成员的支持。