VIRUS EXPRESSION SYSTEMS IN VECTOR BIOLOGY

蚊媒疾病的重要性继续增加，因为 人类和动物的病原体。这些疾病中的许多仍然存在 在世界许多地区难以控制或死灰复燃。小说 迫切需要控制策略来控制这些重要的 疾病。这项研究的总体目标是开发新的控制 以媒介或媒介-病原体界面为目标的策略 中断病媒传播病原体的循环。新的 病媒-病原体相互作用和病媒分子的信息 生物学将使用病毒转导和表达系统来获得， 这一新信息可能被利用来控制媒介传播的 疾病。 辛德比斯(甲型病毒，AV)和伊蚊浓核增多症(DNV)病毒 已经为埃及伊蚊开发了转导系统。 还将开发AV和DNV表达系统，用于表达和 冈比亚按蚊的基因敲除。这些系统将用于 体内感兴趣基因(GOI)的生物学特征 医学上重要的传播者。经鉴定的基因和基因产品 年项目1和2中疟疾和丝虫病传播的决定因素 这一TDRU方案和其他方案将在伊蚊体内得到表征。 和按蚊媒介。 除转导外，DNV系统还具有生防潜力。 胆小鬼的能力。登革热病毒在人体内非常稳定 并且是特定于目标的。DNV系统的潜力 感染和转导改变载体能力或载体的基因 容量将会确定。待研究的基因包括昆虫- 特殊毒素，保幼激素酯酶，伊蚊头肽I， 它规范了寻找宿主的行为。这种效应器的转导 进入载体种群的分子可以提供新的控制机制 用于病媒传播的疾病。

Mosquito-borne diseases continue to increase in significance as pathogens of humans and animals. Many of these diseases remain intractable to control or resurgent in many areas of the world. Novel control strategies are desperately needed for control of these important diseases. The overall goal of this research is to develop novel control strategies that target the vector or vector-pathogen interface to interrupt the cycle of transmission of vector-borne pathogens. New information concerning vector-pathogen interactions and vector molecular biology will be derived using virus transduction and expression systems, and this new information may be exploited to control vector-borne diseases. Sindbis (Alphavirus, AV) and Aedes densonucleosis (DNV)-based virus transduction systems have been developed for Aedes aegypti mosquitoes. AV and DNV expression systems will also be developed for expression and knock out of genes in Anopheles gambiae. These systems will be used to characterize biologically genes of interest (GOIs) in vivo in the medically important vectors. Genes and gene products identified as determinants of malaria and filaria transmission in Projects 1 and 2 of this TDRU proposal and by others will be characterized in vivo in Aedes and Anopheles vectors. The DNV systems possess biocontrol potential in addition to transduction capability of GOls. Densoviruses are extremely stable in the environment and are target specific. The potential for DNV systems to infect and to transduce genes that alter vector competence or vectorial capacity will be determined. Genes to be investigated include insect- specific toxin, juvenile hormone esterase, and Aedes headpeptide I, which regulates host seeking behavior. Transduction of such effector molecules into vector populations could provide novel control mechanisms for vector-borne diseases.