Core--Analytical and morphological

描述：(改编自申请者摘要) 分析和形态核心的目的将是集中 以下技术：分析化学、酶联免疫分析 (ELISA)、放射免疫分析(RIA)、Western印迹分析和受体结合 化验。将使用分析化学来确定亚硝酸盐、硝酸盐和 羟基脯氨酸。将采用ELISA法和RIA法对血浆进行检测 和组织水平的四肽Ac-SDKP，前列腺素，血管活性 多肽、环腺苷和一磷酸鸟苷。Wester印迹分析 将进行金属蛋白酶-2(MMP2)和MMP9的检测。 将用凝胶电泳法测定金属蛋白酶(MMP2和MMP9) 活动。EMSA将被用来评估核激活 转录因子核因子-kappaβ。对于形态测量分析，岩芯Bi. 将准备组织载玻片(即冷冻切片、包埋和 组织学和免疫组织化学染色)用于测量 心肌梗死面积、心肌细胞大小、毛细血管密度、间质 血管周胶原1、3型、间质胶原体积 细胞分裂、细胞凋亡和坏死。最后，体外定量 放射自显影和荧光/共聚焦显微镜将用于 放射受体结合分析及其解剖和细胞定位 膜结合受体、胞内受体和核受体。

DESCRIPTION: (adapted from applicant's abstract) The purpose of the Analytical and Morphological Core will be to centralize the following techniques: analytical chemistry, enzyme-linked immunoassays (ELISA), radioimmunoassays (RIA), Western blot analysis, and receptor binding assay. Analytical chemistry will be used to determine nitrites, nitrates and hydroxyproline. ELISA and RIA will be utilized for the measurement of plasma and tissue levels of the tetrapeptide Ac-SDKP, prostanoids, vasoactive peptide, cyclic adenosine and guanosine monophosphates. Wester blot analysis will be performed to measure metalloproteinase-2 (MMP-2) and MMP-9. Zymography will be used to determine metalloproteinase (MMP-2 and MMP-9) activities. EMSA will be utilized to assess activation of the nuclear transcription factor NF-kappa beta. For the morphometric analyses, Core Bi will prepare tissue slides (i.e., cryostat sectioning, embedding and histological and immunohistochemical staining) for the measurement of myocardial infarct size, cardiomyocyte size, capillary density, interstitial and perivascular collagen types 1 and 3, interstitial collagen volume fraction, apoptosis and necrosis. Finally, Quantitative in vitro autoradiography and fluorescnece/confocal microscopy will be utilized for radioreceptor binding assays and anatomical and cellular localization of membrane-bound, intracellular and nuclear receptors.