Mussel-Inspired Protective Coatings

描述（由申请人提供）：贻贝产生外化的肌腱，称为基底线，这些肌腱共同起固定作用。粗丝表面有一层薄薄的保护角质层（5-20厘米），可以防止沙子、化学侵蚀和微生物降解的磨损，但它足够灵活，可以承受纤维性粗丝芯0.7的张力。Mfp-1是基底包被的主要结构蛋白。它也被商业上用作通用的细胞和组织附着因子，并且正在开发作为聚合物附着在表面的仿生系绳。本提案旨在更好地了解mfp-1在基材涂层中的分布，性质和加工。这被认为是智能合成和生物医学材料应用类似物的必要条件。研究目标包括：1)mfp-1在涂层和足部斑驳颗粒中的免疫定位；2)元素组成与涂层纳米力学性能的映射；利用电化学螯合标度测定了4种不同贻贝（Mytilus galloprovincialis、m.c oranus、Modiolus Modiolus和Septifer bifurcatus） fp-ls的金属结合亲合力；4)利用石英晶体微天平在耗散模式下研究了吸附fp-ls的金属诱导崩解动力学。在第3年和第4年，由mfp-1组成的仿生涂层将通过溶剂蒸发、凝聚和受控的自组装过程在体外制备。所有薄膜都将通过宏观和纳米力学测试进行评估，并检查其对各种生化，热和机械损伤的抵抗力。

DESCRIPTION (provided by applicant): Mussels produce externalized tendons called byssal threads, which collectively serve as holdfasts. Byssal threads are coated with a thin protective cuticle (5-20 qm) that shields against abrasion by sand, chemical erosion, and microbial degradation, yet is flexible enough to accommodate strains of 0.7 by the fibrous byssal core. Mfp-1 is the primary structural protein of the byssal coating. It is also used commercially as a universal cell and tissue attachment factor and is being developed as a biomimetic tether for polymer attachment to surfaces. The present proposal aims to better understand the disposition, properties, and processing of mfp-1 in the byssal coating. This is considered essential for the intelligent synthesis and use of analogues for biomedical materials applications. Research aims include 1) the immunolocalization of mfp-1 in the mottled granules of the coating and foot; 2) mapping the elemental composition with the nanomechanical properties of the coating; measurement of metal binding affinities of fp-ls from four different mussel species (Mytilus galloprovincialis, M. californianus, Modiolus modiolus and Septifer bifurcatus) using an electrochemical chelate scale, and 4) investigating metal-induced collapse kinetics in adsorbed fp-ls by quartz crystal microbalance analysis in dissipation mode. In years 3 and 4, biomimetic coatings comprised of mfp-1 with and without added metal will be fabricated in vitro by solvent evaporation, coacervation, and controlled self-assembly processes. All films will be evaluated by macro- and nanomechanical tests, and their resistance to a diverse range of biochemical, thermal, and mechanical insults will be examined.