Mussel-Inspired Protective Coatings

受贻贝启发的防护涂层

• 批准号: 7036603
• 负责人: JOHN HERBERT WAITE
• 金额: $ 32.13万
• 依托单位: UNIVERSITY OF CALIFORNIA SANTA BARBARA
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-02 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7036603
• 关键词: Bivalvia; biomechanics; biomimetics; granule; iron; polymerase chain reaction; protein localization; protein structure function; scanning electron microscopy; surface coating; transmission electron microscopy

DESCRIPTION (provided by applicant): Mussels produce externalized tendons called byssal threads, which collectively serve as holdfasts. Byssal threads are coated with a thin protective cuticle (5-20 qm) that shields against abrasion by sand, chemical erosion, and microbial degradation, yet is flexible enough to accommodate strains of 0.7 by the fibrous byssal core. Mfp-1 is the primary structural protein of the byssal coating. It is also used commercially as a universal cell and tissue attachment factor and is being developed as a biomimetic tether for polymer attachment to surfaces. The present proposal aims to better understand the disposition, properties, and processing of mfp-1 in the byssal coating. This is considered essential for the intelligent synthesis and use of analogues for biomedical materials applications. Research aims include 1) the immunolocalization of mfp-1 in the mottled granules of the coating and foot; 2) mapping the elemental composition with the nanomechanical properties of the coating; measurement of metal binding affinities of fp-ls from four different mussel species (Mytilus galloprovincialis, M. californianus, Modiolus modiolus and Septifer bifurcatus) using an electrochemical chelate scale, and 4) investigating metal-induced collapse kinetics in adsorbed fp-ls by quartz crystal microbalance analysis in dissipation mode. In years 3 and 4, biomimetic coatings comprised of mfp-1 with and without added metal will be fabricated in vitro by solvent evaporation, coacervation, and controlled self-assembly processes. All films will be evaluated by macro- and nanomechanical tests, and their resistance to a diverse range of biochemical, thermal, and mechanical insults will be examined.

描述(由申请人提供)：贻贝产生外化的肌腱，称为肌腱线，这些肌腱共同充当禁食。双面丝线覆盖有一层薄薄的保护性角质层(5-20QM)，可防止沙子、化学侵蚀和微生物降解的磨损，但具有足够的灵活性，可以容纳纤维状角质层核心的0.7应变。MFP-1是该涂层的主要结构蛋白。它还被用作一种通用的细胞和组织附着因子，并正在被开发为一种仿生系绳，用于聚合物在表面的附着。本建议旨在更好地了解MFP-1在粉末涂料中的配置、性质和工艺。这被认为是生物医学材料应用中智能合成和使用类似物所必需的。研究目标包括1)MFP-1在涂层和足部斑驳颗粒中的免疫定位；2)将元素组成与涂层的纳米机械性能进行映射；使用电化学螯合标尺测量四种不同贻贝(紫贻贝、贝壳贻贝、紫贻贝和七彩贻贝)的FP-1与金属的结合亲和力；以及4)以耗散模式通过石英晶体微天平分析研究金属诱导吸附的FP-LS的崩塌动力学。在第三年和第四年，将通过溶剂挥发、凝聚和受控自组装过程在体外制备由添加金属和不添加金属的MFP-1组成的仿生涂层。所有的薄膜都将通过宏观和纳米机械测试进行评估，并将检查它们对各种生化、热和机械损伤的耐受性。

项目成果

Mussel adhesive protein provides cohesive matrix for collagen type-1α.

• DOI: 10.1016/j.biomaterials.2015.01.033
• 发表时间: 2015-05
• 期刊: BIOMATERIALS
• 影响因子: 14
• 作者: Rodriguez, Nadine R. Martinez;Das, Saurabh;Kaufman, Yair;Wei, Wei;Israelachvili, Jacob N.;Waite, J. Herbert
• 通讯作者: Waite, J. Herbert

Proteins in load-bearing junctions: the histidine-rich metal-binding protein of mussel byssus.

承载连接中的蛋白质：贻贝足丝富含组氨酸的金属结合蛋白。

• DOI: 10.1021/bi061677n
• 发表时间: 2006
• 期刊: Biochemistry
• 影响因子: 2.9
• 作者: Zhao,Hua;Waite,JHerbert
• 通讯作者: Waite,JHerbert

Stiff coatings on compliant biofibers: the cuticle of Mytilus californianus byssal threads.

• DOI: 10.1021/bi900018m
• 发表时间: 2009-03-31
• 期刊: BIOCHEMISTRY
• 影响因子: 2.9
• 作者: Holten-Andersen, Niels;Zhao, Hua;Waite, J. Herbert
• 通讯作者: Waite, J. Herbert

Fluorescence Investigations into Complex Coacervation between Polyvinylimidazole and Sodium Alginate.

• DOI: 10.1021/ma802174t
• 发表时间: 2009-03-24
• 期刊: MACROMOLECULES
• 影响因子: 5.5
• 作者: Srivastava, Aasheesh;Waite, J. Herbert;Stucky, Galen D.;Mikhailovsky, Alexander
• 通讯作者: Mikhailovsky, Alexander

Iron-clad fibers: a metal-based biological strategy for hard flexible coatings.

• DOI: 10.1126/science.1181044
• 发表时间: 2010-04-09
• 期刊: Science (New York, N.Y.)
• 作者: Harrington MJ;Masic A;Holten-Andersen N;Waite JH;Fratzl P
• 通讯作者: Fratzl P