DESIGNED SCAFFOLD ARCHITECTURE AFFECTS BONE REGENERATION

描述：(逐字)大量研究表明，这种生物材料 支架结构可以显著影响组织的结果 通过影响血管形成、组织内生和组织生长的工程治疗 最终实现组织结构功能。能够精确地设计和 制造生物材料支架将增强对支架如何 影响组织再生并导致优化的生物材料支架 组织工程学。 我们最近开发了基于图像的计算技术来设计 制备具有特定复合体的羟基磷灰石陶瓷支架 内部显微组织和外部形状。初步研究是执行一项 对脚手架设计如何影响进行更严格的调查 小型猪下颌骨的血管化、组织植入和组织功能 模特。本研究有三个具体目标： 具体目标1：验证脚手架架构对早期的影响 血管化与组织基质沉积 具体目标2：验证支架结构对长期骨骼的影响 结构和机械刚度 具体目标3：验证早期血管形成和矿化导致 长期的骨形成和足够的机械硬度 三种脚手架设计，正交圆支撑，正交圆 孔洞，和一个正交的三角形孔洞，将在三个不同的 羟基磷灰石(HA)直径(3个设计x 3个扰动=9个不同 脚手架)。这些脚手架将被用来测试我们在 小型猪下颌骨模型。动物将在2周和6周时被宰杀进行检查 内支架结构对细胞的短期影响 分化和组织沉积。动物将在18岁和52岁时被献祭 几周时间检查长期内部支架结构对组织的影响 结构和机械功能。这项研究的结果将有助于我们 通过计算设计优化生物材料支架性能以提高 通过组织工程治疗再生组织结构和功能。

DESCRIPTION: (verbatim) Numerous studies have indicated that the biomaterial scaffold architecture can significantly influence the outcome of tissue engineering treatments through effects on vascularization, tissue ingrowth and final tissue structure function. The ability to precisely design and manufacture biomaterial scaffolds would enhance understanding of how scaffolds influence tissue regeneration and lead to optimized biomaterial scaffolds for tissue engineering. We have recently developed image based computational techniques for designing and manufacturing hydroxyapatite ceramic scaffolds with specific complex internal microstructure and external shape. A preliminary study is to perform a more rigorous investigation into how scaffold design influences vascularization, tissue ingrowth, and tissue function in a minipig mandible model. The present study has three specific aims: Specific Aim 1: Verify that scaffold architecture influences early vascularization and tissue matrix deposition Specific Aim 2: Verify that scaffold architecture influences long-term bone structure and mechanical stiffness Specific Aim 3: Verify that early vascularization and mineralization lead to long-term bone formation and sufficient mechanical stiffness Three scaffold designs, an orthogonal circular strut, an orthogonal circular pore, and an orthogonal triangular pore, will be constructed at three different diameters from hydroxyapatite (HA) (3 designs x 3 perturbations = 9 different scaffolds). These scaffolds will be used to test our specific aims in the minipig mandible model. Animals will be sacrificed at 2 and 6 weeks to examine the short-term effects of internal scaffold architecture on cell differentiation and tissue deposition. Animals will be sacrificed at 18 and 52 weeks to examine long-term internal scaffold architecture influence on tissue structure and mechanical function. Results from this study will assist us in optimizing biomaterial scaffold performance via computational design to enhance regenerate tissue structure and function via tissue engineering treatments.