STRUCTURAL STUDIES OF CYTOCHROME P450

细胞色素P450是一种参与解毒的酶 进程。细菌酶已经被提纯到均一，并且 在SDS-PAA凝胶上以100 kDa的蛋白质形式运行。布莱克博士和同事们 已经能够获得小的三维晶体，这些晶体 不够大，无法进行X射线结晶学分析。 超速离心实验表明，轴比不寻常。 这个分子是7：1。为任何结构工作提供便利，并 为了验证轴比，布莱克博士来到我们的实验室，研究 用电子冷冻显微镜观察蛋白质。初步的图像看起来 支撑拉长形状的模型。此外，这一点 众所周知，水溶性蛋白质与脂质相互作用，可能是一种 外周膜蛋白。因此，实验已经开始。 使脂质单层上的细胞色素P450结晶。

Cytochrome P450 is an enzyme involved in the detoxification process. The bacterial enzyme has been purified to homogeneity and runs as a 100 kDa protein on SDS-PAA gels. Dr. Black and co-workers have been able to obtain small three-dimensional crystals which are not sufficiently large for x-ray crystallographic analysis. Ultracentrifugation experiments have indicated an unusual axial ratio of 7:1 for this molecule. To facilitate any structural work and to verify the axial ratio, Dr. Black visited our lab to investigate the protein by electron cryo-microscopy. Preliminary images seem to support the model of an elongated shape. In addition, this water-soluble protein is known to interact with lipids, perhaps as an peripheral membrane protein. Experiments have therefore been started to crystallize the cytochrome P450 on lipid monolayers.