A Novel Predictor of Nephrolithiasis

描述（由申请人提供）：拟议研究的总体科学和商业目标是开发一种可以预测肾结石患者肾结石复发的临床检测方法。由于目前可用的诊断工具无法预测谁会患上复发性肾结石，那些注定会复发的人将受益于能够提前识别他们的检测。我们建议开发western blotting和ELISA方法来测量各种形式的尿蛋白，这是一种已知的草酸钙结晶抑制剂。初步研究表明，与单独测量结石风险化学物质相比，测量这种蛋白质的一种形式与标准结石风险化学物质相结合，可以提高检测结石患者的灵敏度。我们将对随机抽取的50名结石患者和50名非结石患者的尿液进行化验。无论是单独使用还是与标准结石风险化学试剂结合使用，我们都将确定最能区分结石形成者与非结石形成者的检测方法。在II期，我们将前瞻性地测试这些测量是否可以识别有复发风险的肾结石患者。

DESCRIPTION (provided by applicant): The overall scientific and commercial objective of the proposed research is to develop a clinical assay that can predict the recurrence of kidney stones in patients with nephrolithiasis. Since currently available diagnostic tools cannot predict who will develop recurrent kidney stones, those destined to recur would benefit from tests that could identify them in advance. We propose to develop western blotting and ELISA methods to measure various forms of a urine protein that is a known inhibitor of calcium oxalate crystallization. Preliminary studies have shown that measurement of one form of this protein combined with standard stone risk chemistries improves the sensitivity of detection of stone formers compared with stone risk chemistries alone. We will perform the assays on urine from a random sample of 50 stone formers and 50 non-stone formers. We will identify the assay(s) that best discriminate stone formers from non-stone formers, either when used alone or combined with standard stone risk chemistries. In Phase II we will prospectively test whether these measurements can identify kidney stone patients at risk for recurrence.