TRICHLOROETHYLENE CAPTURE IMMUNOASSAY

描述(由申请人提供)：卤化溶剂，包括氯乙烯、三氯乙烯、1，2-二氯乙烷和氯仿，是超级基金地点的一个重要污染源。其中最重要的卤化溶剂是三氯乙烯(TCE)。三氯乙烯是一种商品溶剂；仅美国的产量就高达30万吨/年。三氯乙烯的药理和毒理学已被广泛研究。三氯乙烯是一种致癌物质和一种肝脏毒素。TCE也是一种无处不在的环境污染物。人类接触三氯乙烯的主要来源是在工作场所和通过受污染的土壤、沉积物和地下水；特别是在危险废物场地附近。目前可用的TCE测定方法通常是基于气相色谱分析的。这些方法需要专用仪器，不容易适应现场或快速反应应用。在现场、高通量和/或现场监测方面，酶免疫分析方法在简单、快速和成本方面比基于仪器的方法具有显著的优势。该计划的目标是开发对环境重要和超级基金的关键卤代溶剂的免疫分析方法。该项目的目标是开发一种三氯乙烯捕获免疫分析方法，该方法将：1)用于危险废物场地监测；2)作为概念验证原型，用于开发卤化溶剂类环境污染物的捕获策略免疫分析方法。

DESCRIPTION (provided by the applicant): The halogenated solvents, which include vinyl chloride, trichloroethylene, 1,2-dichloroethane and chloroform, are a significant source of contamination at Superfund Sites. Among the most important of the halogenated solvents is trichloroethylene (TCE). TCE is a commodity solvent; production levels in the United States alone have been as high as 300,000 tons/year. The pharmacology and toxicology of TCE have been extensively studied. TCE is a carcinogen and a hepatic toxin. TCE is also a ubiquitous environmental contaminant. The primary sources of human exposure to TCE are in the workplace and through contaminated soil, sediment and groundwater; especially near hazardous waste sites. The currently available methods for TCE determination are typically based on Gas Chromatographic analysis. These methods require dedicated instrumentation and are not readily adapted to field or quick response applications. Enzyme ImmunoAssay has repeatedly been demonstrated to have significant advantages over instrument based methods in terms of simplicity, speed and cost for field, high throughput and/or worksite monitoring. The goal of this PROGRAM is the development of immunoassays for the environmentally important and superfund critical halogenated solvents. The goal of this PROJECT is the development of a TCE Capture Immunoassay which will: 1) be used for hazardous waste site monitoring and 2) serve as the proof-of-concept prototype for the development of Capture strategy immunoassays for the halogenated solvent class of environmental contaminants.