MASS SPECTRAL SEQUENCING OF CARBOHYDRATES

描述：(改编自申请者的摘要)这项计划的长远目标 建议的努力是理解与以下相关的结构-功能因素 分子糖基化。具体目标是针对分析问题 在两个关键领域：加强样本捕获和检测的方法 来自生物提取物，以及促进分析物表征的策略 使用改进的离子捕获(MSN)仪器。考虑到最多的 我们讨论了糖蛋白功能中的令人沮丧的问题，例如，多营养 一种创新的遗传方法，首先建立蛋白质组-糖蛋白数据 以简单的动物模型秀丽线虫为基础。后一种努力 由两个将提供糖基转移酶基因的用户组支持 与视觉和化学表型相关的突变体和基因敲除，以及 “内部”遗传学家进行咨询。 为了确保在这些发展框架内有一个有效的重点，我们 另外总结两个用户组的持续应用程序；具有感染力 疾病和糖结合抗原呈递。这些“焦点小组”带来了 从分离和纯化到分析挑战的呼吸 结构表征。团体活动不代表直接财政 给这一应用程序带来负担，并使我们能够为非常重要的问题做出贡献 在免疫学方面。 因此，提案的“具体目标”被安排在标题下。 “化学修饰”(用于增强隔离和检测)，以及 “扩展仪器的能力，”(通过修改离子陷阱质量 光谱仪)。理解功能的第三个目的是提出一种组合基因 和结构方法，“定义糖生物学的功能”，通过建立 使用第一个具有已定义基因的动物模型的蛋白质组-糖蛋白数据库 序列和对细胞谱系的充分理解。 为这些活动提供支持的工作人员在每项活动中都带来了丰富的经验 在本申请中讨论的具有代表性的学科； 仪器、碳水化合物化学、蛋白质组学和糖组学。 此外，为了获得直接咨询和后备支持，纽约大学 汉普郡有一家很好的电子和机械商店，在附近的 建筑，由线虫遗传学家NASA(约翰·柯林斯博士)支持，他 提供蠕虫培养、孵化时间和遗传理解，以及 重要的是，一群化学研究生似乎拥有一种 对仪器和生物学的兴趣，他们的培训得到了 部门。

DESCRIPTION: (Adapted from applicant's abstract) The long range goal of this proposed effort is to understand the structure-functional factors related to molecular glycosylation. The specific aims are directed at analytical problems in two critical areas: methods that will enhance sample capture and detection from biological extracts, and strategies that advance analyte characterization with improved ion trapping (MSn) instrumentation. To consider the most frustrating problem in glycoprotein function, e.g., pleiotrophism, we discuss an innovative genetic approach that begins by building a proteome-glycome data base with the simple animal model, Caenorhabditis elegans. This latter effort is supported by two user groups that will provide glycosyltransferase gene mutants and knockouts to correlate visual and chemical phenotypes, and an 'in-house' geneticist for consultation. To insure an effective focus within the framework of these developments, we additionally summarize continuing applications with two user groups; infectious diseases and glycoconjugate antigen presentation. These "Focus Groups" bring the breath of analytical challenges from isolation and purification to structural characterization. The group activities represent no direct fiscal burden to this application and allow us to contribute to very important issues in immunology. Thus, the "Specific Aims" of the proposal are organized under the heading "Chemical Modifications," (for enhanced isolation and detection), and "Extending Instrumental Capabilities," (by modifications to an ion trap mass spectrometer). A third aim to understand function proposes a combined genetic and structural approach, "Defining Function in Glycobiology," by building a proteome-glycome data base using the first animal model with a defined gene sequence and fully understood cell lineage. The staff supporting for these activities bring considerable experience in each of their representative disciplines discussed in this application; instrumentation, carbohydrate chemistry, proteomics, and glycomics. Additionally, for direct consultation and backup support the University of New Hampshire has an excellent electronics and machine shop in an adjacent building, supported by NASA, a C. elegans geneticist, (Dr. John Collins), who provides worm cultures, incubator time, and genetic understanding, and importantly, a group of chemistry graduate students who seem to possess an appetite for instrumentation and biology whose training is supported by the Department.