A massive attack on how messenger RNA sequence specifies condition-dependent RNA fate

3‘-非翻译区(3’-UTRs)是参与翻译、定位和稳定性的非编码序列。然而，这些功能涉及的确切基序在很大程度上仍不清楚。这个项目的目标是利用模式生物酿酒酵母来了解3‘-UTR序列的作用。该项目将使用生物信息学、转录组学和合成生物学的方法来创建数千个嵌合的mRNA序列，并在多种生理和应激条件下对它们进行测试，包括测量mRNA的产生、加工和衰退。获得的数据可以用来识别调控信号，例如mRNAs非翻译区的序列基序，并建立3‘-UTR序列在基因表达调控和胁迫反应中功能的连贯模型。本项目的结果不仅将影响我们对芽期酵母胁迫反应的理解，也将增加我们对转录后基因表达调控的了解。它们可能有助于我们更好地了解和靶向病原真菌物种，或者成为类似研究的基础，包括哺乳动物的发育和病理学，以及微调芽殖酵母中的蛋白质表达以用于制药目的。

3'-untranslated regions (3'-UTRs) of mRNA are non-coding sequences that are involved in mRNA translation, localization and stability. However, the exact motifs involved in those functions remain largely unknown. The goal of this project is to understand the role of 3'-UTR sequences using model organism Saccharomyces cerevisiae.The project will use bioinformatics, transcriptomics and synthetic biology approaches to create thousands of chimeric mRNA sequences and to test them in multiple physiological and stress conditions, including measurements of mRNA production, processing, and decay. The obtained data can then be used to identify regulatory signals, for example sequence motifs in the untranslated regions of mRNAs and to create a coherent model of the function of 3'-UTR sequences in gene expression regulation and stress response.The results of this project will not only impact our understanding of stress response in budding yeast, but also increase our knowledge of post-transcriptional gene expression regulation. They might help us better understand and target pathogenic fungi species or become a foundation for similar studies, including mammalian development and pathology, as well as fine-tune protein expression in budding yeast for pharmaceutical purposes.