Media for Capillary and Micro-Chip Based Electrophoresis

说明(申请人提供)：续期建议书的具体目的 介绍了一种新型分离介质的开发和优化。 DNA毛细管/芯片(阵列)电泳法。基于以下内容的概念模型 DNA链动力学和聚合物物理的基本原理 借助聚合物合成，已经开发并正在进行测试。我们 建议完成测试，设计新的分离介质，基于 模型，并将我们的分离媒体付诸实践。 聚合物分离介质概念模型的实质是 开发和测试得非常好。基于这一模型，分离 介质应为中性、亲水性和稳定性。它也应该有 表面活性特性。更重要的是，为了实现更快的运行 随着时间的推移，聚合物链应该更长，这样同样的障碍就可以 用较少的聚合物产生，这意味着较少的 需要聚合物才能产生相同的有效目数。此外，在 以分离较大尺寸的片段(例如，DNA；但不限于DNA 单独)，聚合物链纠缠时间应尽可能长(因此 如模拟化学交联凝胶)。我们已经做了什么，应该做什么 是通过使用一组 测试实验，因为有合成限制来创建一个理想的 分离介质。此外，不存在量化的理论处理方法 这种复杂的水平，在实践中，大多数聚合物不能很好地混合。因此，通过 这种新的概念模型，希望能够为我们提供适当的手段 改进任何现有和未来的毛细管分离介质 电泳法。应用生物系统在我们的一个更多的 易于获得的分离介质已经表明，它比 ABI目前使用的商业分离介质。

DESCRIPTION (provided by applicant): The specific aim of the renewal proposal deals with the development and the optimization of better separation media for DNA capillary/microchip (array) electrophoresis. A conceptual model based on the DNA chain dynamics and fundamental principles of polymer physics, together with the aid of polymer synthesis, has been developed and is being tested. We propose to complete the tests, to design new separation media based on the model, and to put our separation media into practice. The essence of the conceptual model on polymer separation medium has been developed and tested out extremely well. Based on this model, the separation medium should be neutral, hydrophilic, and stable. It should also have surface-active properties. More importantly, in order to achieve a faster run time, the polymer chains should be more extended so that the same obstacles can be created with lesser amounts of polymers, implying that a lesser amount of polymers will be needed to create the same effective mesh size. Furthermore, in order to separate larger size fragments (e.g., DNAs; though not limited to DNAs alone), the polymer chain entanglement times should be as long as possible (so as to simulate a chemically cross-linked gel). What we have done and shall do is to demonstrate definitively the concepts of this model by using a set of test experiments since there are synthetic limitations to create an ideal separation medium. Furthermore, no quantitative theoretical treatment exists at this complex level, and in practice, most polymers do not mix well. Thus, with this new conceptual model, we hope to be able to provide the appropriate means to improve any existing and future separation media for capillary electrophoresis. Preliminary tests by Applied Biosystems on one of our more readily accessible separation media have already shown that it is better than the commercial separation medium currently being used by ABI.