Liquid Chormatograph Mass Spectrometer (LC-MS)

该提案要求提供资金购买液相色谱质谱仪（LC-MS），以支持五个主要用户（所有NIH资助）和两个次要用户（一个NIH资助）的研究。所有主要和次要用户都是特拉华州大学化学和生物化学系的教师。该仪器将被放置在一个开放的实验室在监督下的部门质谱设施。非预约用户可以选择几种预先建立的方法之一，这些方法专为快速样品周转而设计。更复杂的实验将在质谱设施人员的协助下进行，并将以最大限度地减少对步入式使用的影响的方式进行安排。拟议的LC-MS仪器将促进几个正在进行的研究项目。这些项目包括：（Colman）新的亲和标记、化学修饰的酶和纯化的修饰肽和突变酶的表征;（Koh）用小分子选择性调节基因表达和用于基因表达的时间和空间控制的光激活基因表达;（Mueller）RNA修饰、假尿苷酶和4-硫代尿苷生成的酶学;（Riordan）乙酰辅酶A合酶的模型研究;（Thorpe）黄素蛋白在脂肪酸代谢中的作用、酰基辅酶A脱氢酶和脂肪酸β-氧化的相关酶以及巯基氧化酶的催化作用。

This proposal requests funds to purchase a liquid chromatograph mass spectrometer (LC-MS) to support the research of five major users (all NIH funded) and two minor users (one NIH funded). All major and minor users are faculty in the chemistry and biochemistry department at the University of Delaware. The instrument will be placed in an open- access laboratory under the supervision of the departmental mass spectrometry facility. Walk-in users will be able to choose one of several pre-established methods that are designed for fast sample turn around. More sophisticated experiments will be performed with assistance from personnel in the mass spectrometry facility and will be scheduled in a manner that minimizes the impact upon walk-in use. The proposed LC-MS instrument will facilitate several ongoing research projects. These projects include: (Colman) characterization of new affinity labels, chemically modified enzymes and purified modified peptides and mutant enzymes; (Koh) selective regulation of gene expression with small molecules and light activated gene expression for temporal and spatial control of gene expression; (Mueller) enzymology of RNA modification, pseudouridine synthases and 4-thiouridine generation; (Riordan) model studies of acetyl coenzyme A synthase; (Thorpe) flavoproteins in fatty acid metabolism, acyl coenzyme A dehydrogenases and related enzymes of fatty acid beta-oxidation, and sulfhydryl oxidase catalysis.